Oppneheim J D, Owen P, Nachbar M S, Colledge K, Kaplan H S
Immunol Commun. 1977;6(2):167-81. doi: 10.3109/08820137709055809.
Wheat germ agglutinin (WGA) has been shown to react specifically with solubilized I blood group substance, purified from papain treated human erythrocyte membranes. WGA and I react to form an affinity precipitate in immunodiffusion gels, a reaction which can be blocked by the incorporation of N-acetyl glucosamine into the gel. The I material was a strong inhibitor of both anti-I cold hemagglutination and WGA hemagglutination reactions. Utilizing the techniques of crossed immunoelectrophoresis we have clearly established that WGA and anti-I IgM cold antibody are reacting with the same membrane macromolecule (I antigen). WGA was then used in a rocket affinoelectrophoretic assay system to quantitate I substance. The limits of detection in this system was 25 ng.
麦胚凝集素（WGA）已被证明能与从木瓜蛋白酶处理的人红细胞膜中纯化的可溶性I血型物质发生特异性反应。WGA和I在免疫扩散凝胶中反应形成亲和沉淀，该反应可通过在凝胶中加入N-乙酰葡糖胺来阻断。I物质是抗I冷凝集和WGA凝集反应的强抑制剂。利用交叉免疫电泳技术，我们已明确证实WGA和抗I IgM冷抗体与同一膜大分子（I抗原）发生反应。然后，WGA被用于火箭亲和电泳分析系统以定量I物质。该系统的检测限为25纳克。
