Khaskhali M H, Bhanger M I, Khand F D
National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):147-51. doi: 10.1016/0378-4347(95)00338-x.
A simple method for the simultaneous determination of oxalic and citric acids had been developed using reversed-phase HPLC. An aqueous solution containing potassium dihydrogen phosphate (0.25%) and tetrabutylammonium hydrogen sulphate (2.5 mmol) at pH 2.0 was used as mobile phase. Under these conditions both the components were well resolved and detected at 210 nm. The recovery for oxalic and citric acids was 97% and 102%, respectively. The method presented here was applied to urine specimens of a large number of urolithic patients and control subjects. Because of the simplicity of the method its application provides better means of monitoring the concentration of oxalic and citric acids in the formation of renal calculi.
已开发出一种使用反相高效液相色谱法同时测定草酸和柠檬酸的简单方法。将含有磷酸二氢钾（0.25%）和硫酸氢四丁铵（2.5 mmol）、pH值为2.0的水溶液用作流动相。在这些条件下，两种成分都得到了很好的分离，并在210 nm处进行检测。草酸和柠檬酸的回收率分别为97%和102%。本文介绍的方法应用于大量尿路结石患者和对照受试者的尿液标本。由于该方法简单，其应用为监测肾结石形成过程中草酸和柠檬酸的浓度提供了更好的手段。
