Cerami C, Zhang X, Ulrich P, Bianchi M, Tracey K J, Berger B J
Picower Institute for Medical Research, Manhasset, NY 11030, USA.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):71-5. doi: 10.1016/0378-4347(95)00346-0.
A high-performance liquid chromatographic method has been developed for a series of aromatic guanylhydrazones that have demonstrated therapeutic potential as anti-inflammatory agents. The compounds were separated using octadecyl or diisopropyloctyl reversed-phase columns, with an acetonitrile gradient in water containing heptane sulfonate, tetramethylammonium chloride, and phosphoric acid. The method was used to reliably quantify levels of analyte as low as 785 ng/ml, and the detector response was linear to at least 50 micrograms/ml using a 100 microliters injection volume. The assay system was used to determine the basic pharmacokinetics of a lead compound, CNI-1493, from serum concentrations following a single intravenous injection in rats.
已开发出一种高效液相色谱法，用于分析一系列芳香胍腙类化合物，这些化合物已显示出作为抗炎剂的治疗潜力。使用十八烷基或二异丙基辛基反相柱分离这些化合物，流动相为含庚烷磺酸盐、四甲基氯化铵和磷酸的水 - 乙腈梯度溶液。该方法能够可靠地定量低至785 ng/ml的分析物水平，进样体积为100微升时，检测器响应在至少50微克/毫升范围内呈线性。该分析系统用于在大鼠单次静脉注射后，根据血清浓度测定先导化合物CNI - 1493的基本药代动力学。
