Ishimaru S, Itoh M, Hanada K, Tsuchida A, Iwao T, Kajiyama G
First Department of Internal Medicine, Hiroshima University School of Medicine. Japan
Cancer. 1996 Jun 1;77(11):2233-9. doi: 10.1002/(SICI)1097-0142(19960601)77:11<2233::AID-CNCR8>3.0.CO;2-Q.
It is often difficult to distinguish pancreatic carcinoma preoperatively from chronic pancreatitis. Therefore, we have developed a new method of detecting p53 immunoreactivity in cytologic material obtained by endoscopic retrograde pancreatic duct brushing (ERPDB).
Twenty-eight patients with prominent strictures of the main pancreatic duct demonstrated by pancreatography including 20 ductal cell carcinoma and 8 chronic pancreatitis were studied. The ability to distinguish between these two groups preoperatively by conventional cytologic examination was compared with p53 immunocytochemistry using ERPDB:
The sensitivity, specificity, and overall accuracy of conventional cytologic examination in distinguishing ductal cell carcinoma from chronic pancreatitis were 60%, 100%, and 71% respectively. In comparison, the sensitivity, specificity, and overall accuracy of p53 immunocytochemistry in distinguishing were 90%, 100%, and 93%, respectively. The sensitivity of p53 staining of specimens from patients with carcinoma of the body or tail of the pancreas (90%) was the same for those with tumors of the head of the pancreas (90%).
These results suggest that p53 immunocytochemistry using ERPDB in conjunction with conventional cytologic examination can help differentiate ductal cell carcinoma from chronic pancreatitis preoperatively.
术前区分胰腺癌与慢性胰腺炎往往很困难。因此，我们开发了一种新方法，用于检测通过内镜逆行胰管刷检（ERPDB）获取的细胞学材料中的p53免疫反应性。
对28例经胰管造影显示主胰管明显狭窄的患者进行研究，其中包括20例导管细胞癌和8例慢性胰腺炎患者。将通过传统细胞学检查术前区分这两组患者的能力与使用ERPDB的p53免疫细胞化学方法进行比较。
传统细胞学检查区分导管细胞癌与慢性胰腺炎的敏感性、特异性和总体准确率分别为60%、10并分别为90%、100%和93%。胰腺体尾部癌患者标本的p53染色敏感性（90%）与胰头部肿瘤患者相同（90%）。
这些结果表明，使用ERPDB的p53免疫细胞化学结合传统细胞学检查有助于术前区分导管细胞癌与慢性胰腺炎。 0%和71%。相比之下，p53免疫细胞化学区分的敏感性、特异性和总体准确率分
