Chintala S K, Sawaya R, Gokaslan Z L, Rao J S
Department of Neurosurgery, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Cancer Lett. 1996 Jun 5;103(2):201-8. doi: 10.1016/0304-3835(96)04215-2.
We have investigated the effect of integrin antibodies to a well-characterized alpha 5 beta 1 (fibronectin receptor) and to a multi-specific alpha 3 beta 1 (laminin, collagen, and fibronectin receptor), on the expression of matrix metalloproteases and the invasion ability of two human glioblastoma cell lines, SNB19 and U251. Cell adhesion assays indicated that both cell lines adhere to fibronectin, type IV collagen and laminin. Adhesion of cells to fibronectin was inhibited by a RGD peptide. Cells treated with anti-alpha 3 beta 1 or anti-alpha 5 beta 1 antibodies expressed increased levels of MMP-2. An in vitro matrigel assay also showed that the alpha 3 beta 1 antibody-treated cells had greater invasive ability than the controls. Immunofluorescence data showed that glioma cells treated with either anti-alpha 3 beta 1 or anti-alpha 5 beta 1 antibodies expressed diminished alpha 3 beta-1 and alpha 5 beta 1 integrins relative to the controls. The data show that treatment of cells with alpha 3 beta 1 antibody diminishes the integrin expression on the cell surface and increases the MMP-2 activity and invasiveness.
我们研究了整合素抗体对特征明确的α5β1（纤连蛋白受体）和多特异性α3β1（层粘连蛋白、胶原蛋白和纤连蛋白受体）的影响，以及它们对两种人胶质母细胞瘤细胞系SNB19和U251的基质金属蛋白酶表达和侵袭能力的影响。细胞黏附试验表明，这两种细胞系均能黏附于纤连蛋白、IV型胶原蛋白和层粘连蛋白。细胞对纤连蛋白的黏附受到RGD肽的抑制。用抗α3β1或抗α5β1抗体处理的细胞表达的MMP-2水平升高。体外基质胶试验还表明，用α3β1抗体处理的细胞比对照细胞具有更强的侵袭能力。免疫荧光数据显示，与对照相比，用抗α3β1或抗α5β1抗体处理的胶质瘤细胞表达的α3β1和α5β1整合素减少。数据表明，用α3β1抗体处理细胞会减少细胞表面整合素的表达，并增加MMP-2活性和侵袭性。
