Okajima E, Fukuda T, Okita S, Tsutsumi M, Hirao Y, Okajima E, Konishi Y
Department of Oncological Pathology, Nara Medical University, Japan.
Cancer Lett. 1996 Jun 5;103(2):227-31. doi: 10.1016/0304-3835(96)04225-5.
Although superficial bladder cancer, usually presenting as low grade transitional cell carcinomas, are easily resected by transurethral intervention, their frequent recurrence and progression of satage or grade of the recurrent tumors in some cases is a major problem in urology. Deletion of chromosome 9, bands 9p21-22 in bladder cancers including the lowest grade and stage, suggest potential location of candidate tumor suppressor genes. Recently, p16/MTS1 was isolated from 9p21-22 as a multiple tumor suppressor gene, which regulates the cyclin dependent kinase 4 in the G1/S phase of the cell cycle. In the present study, somatic alterations of p16/MTS1 were examined concentrating on histologically defined superficial bladder carcinomas by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) technique using paraffin embedded materials. Infrequent alterations of p16/MTS1 in superficial bladder cancers, one deletion and one silent mutation in 15 cases, were detected. The results suggest that p16/MTS1 mutation is not involved in the development of superficial urinary bladder carcinomas.
尽管浅表性膀胱癌通常表现为低级别移行细胞癌，可通过经尿道干预轻松切除，但它们频繁复发以及某些情况下复发肿瘤的分期或分级进展是泌尿外科的一个主要问题。在包括最低级别和分期的膀胱癌中，9号染色体9p21 - 22带的缺失提示候选肿瘤抑制基因的潜在定位。最近，p16/MTS1从9p21 - 22中分离出来，作为一种多肿瘤抑制基因，它在细胞周期的G1/S期调节细胞周期蛋白依赖性激酶4。在本研究中，使用石蜡包埋材料，通过聚合酶链反应（PCR）-单链构象多态性（SSCP）技术，集中研究组织学定义的浅表性膀胱癌中p16/MTS1的体细胞改变。在浅表性膀胱癌中检测到p16/MTS1的改变不常见，15例中有1例缺失和1例沉默突变。结果表明p16/MTS1突变不参与浅表性膀胱癌的发生发展。
