Honda Y, Takahashi H, Kuroki Y, Akino T, Abe S
Third Department of Internal Medicine, Sapporo Medical University, School of Medicine, Japan.
Chest. 1996 Apr;109(4):1006-9. doi: 10.1378/chest.109.4.1006.
Hydrophilic surfactant proteins, surfactant protein A (SP-A) and surfactant protein D (SP-D), have important roles in modulating the host defense functions in the peripheral airways. It has been reported that cigarette smoke may alter the component and function of pulmonary surfactant. In this study, we determined the contents of SP-A and SP-D in BAL fluids of healthy smokers and nonsmokers by enzyme-linked immunosorbent assay using monoclonal antibodies against each protein. The contents of SP-A and SP-D in BAL fluids were significantly (p<0.05) decreased in smokers compared to those in nonsmokers, although there was no significant difference of total phospholipid content between smokers and nonsmokers. These results suggest that the decreased levels of SP-A and SP-D in smokers may impair the host defense functions of surfactant in the peripheral airways and might have a crucial roles in the development of chronic obstructive lung disease.
亲水性表面活性蛋白，即表面活性蛋白A（SP-A）和表面活性蛋白D（SP-D），在调节外周气道的宿主防御功能中发挥着重要作用。据报道，香烟烟雾可能会改变肺表面活性剂的成分和功能。在本研究中，我们使用针对每种蛋白质的单克隆抗体，通过酶联免疫吸附测定法测定了健康吸烟者和非吸烟者支气管肺泡灌洗（BAL）液中SP-A和SP-D的含量。与非吸烟者相比，吸烟者BAL液中SP-A和SP-D的含量显著降低（p<0.05），尽管吸烟者和非吸烟者之间的总磷脂含量没有显著差异。这些结果表明，吸烟者SP-A和SP-D水平的降低可能会损害外周气道表面活性剂的宿主防御功能，并且可能在慢性阻塞性肺疾病的发展中起关键作用。
