Lewis D C, Meyers K M
Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, Pullman, USA.
Exp Hematol. 1996 May;24(6):696-701.
Canine idiopathic thrombocytopenic purpura (ITP) is clinically analogous to chronic ITP in human beings. The objective of this study was to investigate the pathogenesis of canine ITP by determining whether immunoglobulins bound to the surface of platelets from dogs with ITP (platelet-bound immunoglobulins) were directed against host platelet antigen and whether platelet glycoproteins (GP) IIb and IIIa were target antigens in dogs with ITP. Thirty-two dogs with ITP were studied. Increased platelet-bound immunoglobulin concentrations were detected in 30 cases (94%), and increased concentrations of serum platelet-bindable immunoglobulins were detected in 11 cases (34%). Immunoglobulins eluted from the surface of platelets from dogs with ITP bound to homologous normal canine platelets in 11 of 19 cases (58%). Immunoglobulins against platelet membrane GP IIb and/or IIIa were detected in serum from four of 17 affected dogs. This study provides evidence that immunoglobulins bound to surface of platelets from some dogs with ITP are directed against host platelet antigens and that platelet target antigens are, in some cases, GP IIb and IIIa. This supports the hypothesis that canine ITP is an autoimmune disease, similar to the pathogenesis of chronic ITP in human beings.
犬特发性血小板减少性紫癜（ITP）在临床上与人类慢性ITP相似。本研究的目的是通过确定ITP犬血小板表面结合的免疫球蛋白（血小板结合免疫球蛋白）是否针对宿主血小板抗原，以及血小板糖蛋白（GP）IIb和IIIa是否为ITP犬的靶抗原，来研究犬ITP的发病机制。对32只ITP犬进行了研究。30例（94%）检测到血小板结合免疫球蛋白浓度升高，11例（34%）检测到血清血小板可结合免疫球蛋白浓度升高。19例中有11例（58%）从ITP犬血小板表面洗脱的免疫球蛋白与同源正常犬血小板结合。17只患病犬中有4只血清中检测到针对血小板膜GP IIb和/或IIIa的免疫球蛋白。本研究提供了证据，表明一些ITP犬血小板表面结合的免疫球蛋白针对宿主血小板抗原，并且在某些情况下血小板靶抗原为GP IIb和IIIa。这支持了犬ITP是一种自身免疫性疾病的假说，类似于人类慢性ITP的发病机制。
