Skaggs B A, Alexander J F, Pierson C A, Schweitzer K S, Chun K T, Koegel C, Barbuch R, Bard M
Department of Biology, Indiana University-Purdue University at Indianapolis 46202, USA.
Gene. 1996 Feb 22;169(1):105-9. doi: 10.1016/0378-1119(95)00770-9.
The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants. ERG5 is the putative gene encoding the C-22 sterol desaturase required in ergosterol biosynthesis. The functional gene was localized to a 2.15-kb SacI-EcoRI DNA fragment containing an open reading frame of 538 amino acids (aa). ERG5 contains a 10-aa motif consistent with its role as a cytochrome P-450 (CyP450) enzyme and is similar to a number of mammalian CyP450 enzymes. Gene disruption demonstrates that ERG5 is not essential for cell viability.
通过使用涉及筛选制霉菌素敏感转化体的阴性选择方案，对erg5-1突变进行互补，克隆了酿酒酵母的ERG5基因。ERG5是在麦角固醇生物合成中所需的推定编码C-22固醇去饱和酶的基因。功能基因定位于一个2.15 kb的SacI-EcoRI DNA片段，该片段包含一个538个氨基酸（aa）的开放阅读框。ERG5包含一个10个氨基酸的基序，与其作为细胞色素P-450（CyP450）酶的作用一致，并且与许多哺乳动物的CyP450酶相似。基因破坏表明ERG5对细胞活力不是必需的。
