与大肠杆菌肽基 - tRNA水解酶编码基因同源的微生物基因。

Microbial genes homologous to the peptidyl-tRNA hydrolase-encoding gene of Escherichia coli.

作者信息

De La Vega F M, Galindo J M, Old I G, Guarneros G

机构信息

Departamento de Genética y Biologia Molecular, Centro de Investigación y Estudios Avanzados del I.P.N, México.

出版信息

Gene. 1996 Feb 22;169(1):97-100. doi: 10.1016/0378-1119(95)00823-3.

DOI:10.1016/0378-1119(95)00823-3

PMID:8635758

Abstract

We have cloned and determined the nucleotide (nt) sequences of the genes encoding peptidyl-tRNA hydrolase (Pth) homologues of Salmonella typhi (St) and the Lyme disease spirochaete, Borrelia burgdorferi (Bb). We also completed the nt sequence of a pth homologous gene contained in a Chlamydia trachomatis (Ct) clone identified in the databanks. The open reading frames (ORFs) of the Pth homologues encode putative polypeptides of 194 (St), 188 (Bb) and 194 (Ct) amino acids exhibiting significant identity with Escherichia coli (Ec) Pth. Together with the products of two previously unidentified ORFs from Bacillus subtilis and Saccharomyces cerevisiae, and the recently recognized Haemophilus influenzae and Mycoplasma genitalium pth genes, these seven putative polypeptides and the Ec Pth form a group of homologous basic proteins spanning eubacteria and eukaryota which can be defined by at least three conserved regions. Previously known Ec pth mutations were located in highly conserved residues.

摘要

我们已经克隆并测定了编码伤寒沙门氏菌（St）和莱姆病螺旋体——伯氏疏螺旋体（Bb）的肽基 - tRNA水解酶（Pth）同源物的基因的核苷酸（nt）序列。我们还完成了数据库中鉴定的沙眼衣原体（Ct）克隆中所含pth同源基因的核苷酸序列测定。Pth同源物的开放阅读框（ORF）编码推定的由194个氨基酸（St）、188个氨基酸（Bb）和194个氨基酸（Ct）组成的多肽，它们与大肠杆菌（Ec）的Pth具有显著的同源性。连同来自枯草芽孢杆菌和酿酒酵母的两个先前未鉴定的ORF的产物，以及最近发现的流感嗜血杆菌和生殖支原体的pth基因，这七种推定的多肽和Ec的Pth形成了一组跨越真细菌和真核生物的同源碱性蛋白，它们可由至少三个保守区域来界定。先前已知的Ec pth突变位于高度保守的残基中。