• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

IS1的insE开放阅读框对于共整合体的形成并非必需。

The insE open reading frame of IS1 is not required for formation of cointegrates.

作者信息

Freund E T, Susskind M M

机构信息

Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles 90089-1340, USA.

出版信息

J Bacteriol. 1996 Apr;178(8):2420-3. doi: 10.1128/jb.178.8.2420-2423.1996.

DOI:10.1128/jb.178.8.2420-2423.1996
PMID:8636048
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177955/
Abstract

The role of the insE open reading frame in transposition of IS1 was reexamined by using an insE nonsense mutation that does not alter the amino acid sequence of InsA inhibitor or InsAB transposase. The mutant was active in all strains tested, showing that insE is not essential for formation of cointegrates.

摘要

通过使用不改变InsA抑制剂或InsAB转座酶氨基酸序列的insE无义突变，重新研究了insE开放阅读框在IS1转座中的作用。该突变体在所有测试菌株中均具有活性，表明insE对于共整合体的形成不是必需的。

相似文献

1
The insE open reading frame of IS1 is not required for formation of cointegrates.IS1的insE开放阅读框对于共整合体的形成并非必需。
J Bacteriol. 1996 Apr;178(8):2420-3. doi: 10.1128/jb.178.8.2420-2423.1996.
2
[Study of the role of the insA open reading frame in the IS1 insertion element structure during transposition and resolution of the IS1 mediated cointegrates].[关于insA开放阅读框在IS1介导的共整合体转座和拆分过程中在IS1插入元件结构中的作用的研究]
Mol Biol (Mosk). 1990 Nov-Dec;24(6):1549-61.
3
Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?IS1转座酶InsAB'是高效转座所需的唯一一种由IS1编码的蛋白质吗？
J Bacteriol. 1994 Sep;176(18):5864-7. doi: 10.1128/jb.176.18.5864-5867.1994.
4
Involvement of two domains with helix-turn-helix and zinc finger motifs in the binding of IS1 transposase to terminal inverted repeats.IS1转座酶与末端反向重复序列结合过程中两个具有螺旋-转角-螺旋和锌指基序的结构域的参与情况。
Mol Microbiol. 2004 Jul;53(1):193-202. doi: 10.1111/j.1365-2958.2004.04103.x.
5
Genetic evidence for IS1 transposition regulated by InsA and the delta InsA-B'-InsB species, which is generated by translation from two alternative internal initiation sites and frameshifting.由InsA以及由两个交替内部起始位点翻译和移码产生的δ InsA - B'- InsB物种调控IS1转座的遗传证据。
J Mol Biol. 1994 Jul 1;240(1):52-65. doi: 10.1006/jmbi.1994.1417.
6
Mutational analysis of the open reading frames in the transposable element IS1.转座因子IS1中开放阅读框的突变分析。
Genetics. 1988 Sep;120(1):47-55. doi: 10.1093/genetics/120.1.47.
7
IS1-encoded proteins, InsA and the InsA-B'-InsB transframe protein (transposase): functions deduced from their DNA-binding ability.由插入序列1（IS1）编码的蛋白质InsA和InsA-B'-InsB移码蛋白（转座酶）：从其DNA结合能力推导的功能
Adv Biophys. 1995;31:209-22. doi: 10.1016/0065-227x(95)99393-4.
8
Frequency of IS1-mediated molecular events in different members of the family Enterobacteriaceae.肠杆菌科不同成员中IS1介导的分子事件的频率。
J Bacteriol. 1987 Nov;169(11):4946-9. doi: 10.1128/jb.169.11.4946-4949.1987.
9
[Function of the InsA gene in the IS1 element of the Tn9' transposon: influence of oligonucleotide inserts in the InsA gene on formation of simple insertions and plasmid cointegrates].[Tn9'转座子IS1元件中InsA基因的功能：InsA基因中寡核苷酸插入对简单插入和质粒共整合体形成的影响]
Genetika. 1991 Aug;27(8):1301-15.
10
Overexpression of the multidrug efflux operon acrEF by insertional activation with IS1 or IS10 elements in Salmonella enterica serovar typhimurium DT204 acrB mutants selected with fluoroquinolones.通过用IS1或IS10元件进行插入激活，在经氟喹诺酮类药物筛选的鼠伤寒沙门氏菌DT204 acrB突变体中，多药外排操纵子acrEF的过表达。
Antimicrob Agents Chemother. 2005 Jan;49(1):289-301. doi: 10.1128/AAC.49.1.289-301.2005.

本文引用的文献

1
Genetic analysis of the erf region of the bacteriophage P22 chromosome.噬菌体P22染色体erf区域的遗传分析。
Virology. 1984 Apr 15;134(1):148-60. doi: 10.1016/0042-6822(84)90280-0.
2
Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing.能够产生高水平单链DNA用于快速DNA测序的克隆载体。
Gene. 1984 Feb;27(2):183-91. doi: 10.1016/0378-1119(84)90139-2.
3
Cointegrate formation mediated by Tn9. II. Activity of IS1 is modulated by external DNA sequences.由Tn9介导的共整合体形成。II. IS1的活性受外部DNA序列调控。
J Mol Biol. 1983 Oct 15;170(1):61-91. doi: 10.1016/s0022-2836(83)80227-7.
4
Distribution of the insertion sequence IS1 in gram-negative bacteria.插入序列IS1在革兰氏阴性菌中的分布。
Nature. 1981 Feb 12;289(5798):609-12. doi: 10.1038/289609a0.
5
Insertion element IS1 encodes two structural genes required for its transposition.插入元件IS1编码其转座所需的两个结构基因。
J Mol Biol. 1984 Aug 5;177(2):229-45. doi: 10.1016/0022-2836(84)90454-6.
6
Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor.两类对F类R因子介导的转移抑制不敏感的弗氏菌突变体。
Mol Gen Genet. 1971;111(3):256-64. doi: 10.1007/BF00433110.
7
Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium. II. Genetic evidence for two exclusion systems.鼠伤寒沙门氏菌溶原菌中P22原噬菌体介导的超感染排除。II. 两种排除系统的遗传学证据。
Virology. 1971 Sep;45(3):638-52. doi: 10.1016/0042-6822(71)90178-4.
8
The effects of mutations in the ant promoter of phage P22 depend on context.噬菌体P22抗启动子突变的影响取决于背景。
Genetics. 1988 Oct;120(2):319-27. doi: 10.1093/genetics/120.2.319.
9
Functional promoters created by the insertion of transposable element IS1.通过插入转座因子IS1产生的功能性启动子。
J Mol Biol. 1986 Oct 5;191(3):383-93. doi: 10.1016/0022-2836(86)90134-8.
10
Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.质粒R100和F中的阻遏基因finO：质粒F的组成型转移是由IS3插入F finO引起的。
J Bacteriol. 1987 Feb;169(2):619-23. doi: 10.1128/jb.169.2.619-623.1987.