马氏甲烷八叠球菌Gö1反向囊泡中ATP依赖的H⁺泵活性及膜ATP酶的特性
ATP-dependent H+ -pump activity in inverted vesicles of Methanosarcina mazei Gö1 and characterization of membrane ATPase.
作者信息
Inatomi K I
机构信息
Advanced Technology R & D Center, Mitsubishi Electric Corporation, Hyogo, Japan.
出版信息
J Bacteriol. 1996 Apr;178(8):2424-6. doi: 10.1128/jb.178.8.2424-2426.1996.
Abstract
ATP-dependent H+ -pump activity was found in inverted vesicles of Methanosarcina mazei Gö1 by using acridine orange as a fluorescent probe. The H+ -pump activity specifically required both Mg and sulfite ions, but azide, an inhibitor of F0F1-ATPase, did not inhibit the activity. The membranes prepared from M. mazei also had an Mg-ATPase activity, and at least the presence of vacuolar-type ATPase was detected.
摘要
通过使用吖啶橙作为荧光探针，在马氏甲烷八叠球菌Gö1的内翻囊泡中发现了ATP依赖性H⁺泵活性。H⁺泵活性特别需要镁离子和亚硫酸根离子，但F₀F₁ - ATP酶的抑制剂叠氮化物并不抑制该活性。从马氏甲烷八叠球菌制备的膜也具有Mg - ATP酶活性，并且至少检测到了液泡型ATP酶的存在。
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