针对人生长激素22K和20K同工型的单克隆抗体的特性鉴定。

Characterization of monoclonal antibodies specific for the human growth hormone 22K and 20K isoforms.

作者信息

Mellado M, Rodriguez-Frade J M, Kremer L, Martinez-Alonso C

机构信息

Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Campus Cantoblanco, Universidad Autonoma, Madrid, Spain. jmmelladosamba.cnb.uam.es.

出版信息

J Clin Endocrinol Metab. 1996 Apr;81(4):1613-8. doi: 10.1210/jcem.81.4.8636376.

DOI:10.1210/jcem.81.4.8636376

PMID:8636376

Abstract

We have derived and characterized a set of monoclonal antibodies (mAb) specific for the different human GH (hGH) isoforms. The binding characteristics of each antibody to the hGH isoforms (22K and 20K) were analyzed in direct and competitive immunoassays as well as by Western blot. We studied the effects of these mAb on the biological activity of hGH and showed that they specifically block their respective activities. Using these mAb, we developed several immunoassays that have been applied for the quantitation of the different hGH isoforms in body fluids. Therefore, these mAb may help to unravel the biological function of these variants.

摘要

我们已经获得并鉴定了一组针对不同人生长激素（hGH）同工型的单克隆抗体（mAb）。通过直接和竞争性免疫测定以及蛋白质免疫印迹分析了每种抗体与hGH同工型（22K和20K）的结合特性。我们研究了这些单克隆抗体对hGH生物活性的影响，结果表明它们特异性地阻断了各自的活性。利用这些单克隆抗体，我们开发了几种免疫测定方法，用于定量体液中不同的hGH同工型。因此，这些单克隆抗体可能有助于揭示这些变体的生物学功能。