Gustafsson A, Asman B
Department of Periodontology, Karolinska Institutet, Huddinge, Sweden.
J Clin Periodontol. 1996 Jan;23(1):38-44. doi: 10.1111/j.1600-051x.1996.tb00502.x.
The release of free oxygen radicals and degranulation was studied in neutrophils from 14 patients with adult periodontitis and 14 age- and sex-matched healthy controls. The neutrophils were activated by Fc gamma-receptor stimulation, using Staphylococcus aureus opsonized with gamma globulin. Release of oxygen radicals was measured as luminol-enhanced chemiluminescence. Degranulation was assessed as release of elastase, measured with a specific substrate and as release of lactoferrin measured with ELISA. The neutrophils from the patients showed a significantly higher chemiluminescence and a slightly higher release of elastase, whereas the release of lactoferrin was the same in both groups. In contrast, the ratio between the 2 degranulation products, elastase and lactoferrin, was significantly higher in the group with periodontitis. A flow cytometric analysis of the membrane expression of the adhesion molecules CD 11a, CD 11b, CD 15, CD 16, CD 35 and Mel 14 showed no differences in the median immunofluorescence between the 2 groups. This study showed a more than 2-fold higher release of free oxygen radicals from Fc-gamma-receptor stimulated neutrophils compared with healthy controls, which indicates a specific neutrophil-associated host response in adult periodontitis.
对14例成人牙周炎患者和14名年龄及性别匹配的健康对照者的中性粒细胞进行了游离氧自由基释放和脱颗粒研究。使用经γ球蛋白调理的金黄色葡萄球菌通过Fcγ受体刺激激活中性粒细胞。氧自由基释放以鲁米诺增强化学发光法测定。脱颗粒以弹性蛋白酶释放来评估，用特定底物进行测量，乳铁蛋白释放用ELISA法测量。患者的中性粒细胞显示出显著更高的化学发光和略高的弹性蛋白酶释放，而两组中乳铁蛋白的释放相同。相比之下，牙周炎组中两种脱颗粒产物弹性蛋白酶和乳铁蛋白之间的比率显著更高。对黏附分子CD 11a、CD 11b、CD 15、CD 16、CD 35和Mel 14的膜表达进行流式细胞术分析，结果显示两组之间的中位免疫荧光无差异。这项研究表明，与健康对照相比，Fcγ受体刺激的中性粒细胞释放的游离氧自由基高出两倍多，这表明成人牙周炎中存在特定的中性粒细胞相关宿主反应。
