Gustafsson A, Asman B, Bergström K
Division of Periodontology, Karolinska Institute, Huddinge, Sweden.
Oral Dis. 1997 Sep;3(3):167-71. doi: 10.1111/j.1601-0825.1997.tb00030.x.
To study the response to in vitro priming of peripheral neutrophils from patients with periodontitis compared to healthy controls. Peripheral neutrophils from these patients had shown increased production of oxygen radicals after activation with opsonized bacteria and a difference in priming response could suggest an explanation for this hyperreactivity.
Peripheral neutrophils from a group of patients with periodontitis and from age- and sex-matched controls were preincubated with tumor necrosis factor alpha, lipopolysaccharide (LPS), formyl-methionyl-leucyl-phenylalanine and the tetra peptide arginyl-glycyl-aspartate-serine. After preincubation, the cells were activated with gammaglobulin opsonized-bacteria, i.e., a Fc gamma R-stimulation. The priming effect was assessed as the production of oxygen radicals and as the degranulation or primary granules.
Showed that the patients had a slightly lower responsiveness to priming than had the controls. This difference in priming response was most pronounced when measured as degranulation of primary granules after preincubation with LPS and 20 min of activation.
This study shows no difference in response to priming, with optimal concentrations of inflammatory mediators, between peripheral neutrophils from patients with adult periodontitis and healthy controls.
研究与健康对照相比，牙周炎患者外周血中性粒细胞对体外启动的反应。这些患者的外周血中性粒细胞在用调理过的细菌激活后显示出氧自由基产生增加，启动反应的差异可能为这种高反应性提供一种解释。
将一组牙周炎患者及年龄和性别匹配的对照者的外周血中性粒细胞与肿瘤坏死因子α、脂多糖（LPS）、甲酰甲硫氨酰亮氨酰苯丙氨酸和四肽精氨酰甘氨酰天冬氨酰丝氨酸进行预孵育。预孵育后，用γ球蛋白调理过的细菌激活细胞，即FcγR刺激。启动效应通过氧自由基产生以及脱颗粒或初级颗粒来评估。
显示患者对启动的反应性略低于对照者。当以与LPS预孵育并激活20分钟后初级颗粒的脱颗粒来衡量时，这种启动反应的差异最为明显。
本研究表明，在使用最佳浓度的炎症介质时，成人牙周炎患者与健康对照者的外周血中性粒细胞对启动的反应没有差异。
