Johnson S S, Conder G A
Pharmacia & Upjohn, Inc., Kalamazoo, Michigan 49001, USA.
J Parasitol. 1996 Jun;82(3):492-5.
The jird (Meriones unguiculatus) has been shown to be a useful model host for the cestodes Taenia crassiceps and Echinococcus multilocularis. This report outlines a novel model in which hydrocortisone-treated jirds (0.02% in the feed) are infected with another cestode, Hymenolepis diminuta. Jirds were inoculated with 5 freshly harvested cysticercoids of H. diminuta prior to (day 0, -1, or -5) or after (day 1 or 5) switching to medicated feed; in some cases, jirds were never medicated. On days 7, 14, 21, or 28 postinoculation (PI), jirds were killed by CO2 inhalation and their small intestines were examined for tapeworms. Hymenolepis diminuta established, grew, and developed to the gravid adult state in jirds. They persisted longer in medicated (21 days) than in nonmedicated (7 days) animals, and generally higher levels of infection were obtained when jirds were inoculated immediately prior to switching to medicated feed. Treatment of infected jirds on day 4 or days 4, 5, and 6 PI with selected anthelmintics followed by necropsy on day 7 PI discriminated drugs with known activity against tapeworms from those with little or no activity. This rodent in vivo model should provide a useful adjunct for anthelmintic studies.
已证明肥尾沙鼠（Meriones unguiculatus）是多头带绦虫（Taenia crassiceps）和多房棘球绦虫（Echinococcus multilocularis）的一种有用的模型宿主。本报告概述了一种新型模型，其中用氢化可的松处理过的肥尾沙鼠（饲料中含0.02%）感染另一种绦虫——微小膜壳绦虫（Hymenolepis diminuta）。在切换到含药饲料之前（第0、-1或-5天）或之后（第1或5天），给肥尾沙鼠接种5个新收获的微小膜壳绦虫似囊尾蚴；在某些情况下，肥尾沙鼠从未用药。在接种后（PI）第7、14、21或28天，通过吸入二氧化碳处死肥尾沙鼠，并检查其小肠中的绦虫。微小膜壳绦虫在肥尾沙鼠体内建立、生长并发育至妊娠成虫状态。它们在用药的动物（21天）中比未用药的动物（7天）持续时间更长，并且当肥尾沙鼠在切换到含药饲料之前立即接种时，通常能获得更高的感染水平。在接种后第4天或第4、5和6天用选定的驱虫药治疗感染的肥尾沙鼠，然后在接种后第7天进行尸检，可区分出对绦虫有已知活性的药物和活性很小或无活性的药物。这种啮齿动物体内模型应为驱虫药研究提供一种有用的辅助手段。
