Zamith H P, Paumgartten F J, Speit G
Fundação Oswaldo Cruz, INCQS, Rio de Janeiro, Brazil.
Mutat Res. 1996 May;368(1):15-20. doi: 10.1016/s0165-1218(96)90035-x.
The latex of Christ's Crown (Euphorbia milii var. hislopii, syn. E. splendens var. hislopii) is a highly active plant molluscicide and could be used for snail control to reduce the prevalence of schistosomiasis in endemic areas. In the course of its toxicological evaluation, the mutagenicity of the latex of Euphorbia milii was tested in mammalian cells in vitro and in vivo. Latex was investigated for its capability of inducing gene mutations and chromosome aberrations in V79 cells in the absence and presence of S9-mix. Concentrations up to 800 micrograms/ml neither induced gene mutations at the HPRT locus nor chromosome aberrations. Latex had no effect on the frequencies of chromosome aberrations in the bone marrow of male and female rats at a dose of 1000 mg/kg. The results indicate that latex of E. milii is not mutagenic in mammalian cells in vitro and in vivo and its use as a molluscicide does not pose a mutagenic hazard for humans.
虎刺梅（铁海棠变种希斯洛皮变种，同物异名：猩猩草变种希斯洛皮变种）的乳汁是一种活性很高的植物杀螺剂，可用于控制蜗牛数量，以降低流行地区血吸虫病的发病率。在其毒理学评估过程中，对铁海棠乳汁在体外和体内哺乳动物细胞中的致突变性进行了测试。研究了乳汁在有无S9混合液的情况下诱导V79细胞基因突变和染色体畸变的能力。浓度高达800微克/毫升时，既未在次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）位点诱导基因突变，也未诱导染色体畸变。在剂量为1000毫克/千克时，乳汁对雄性和雌性大鼠骨髓中的染色体畸变频率没有影响。结果表明，铁海棠乳汁在体外和体内哺乳动物细胞中均无致突变性，其作为杀螺剂的使用对人类不存在诱变危害。
