Côté P Y, Levitt P, Parent A
Centre de Recherche en Neurobiologie, Université Laval et Hôpital de l'Enfant-Jésus, Québec, Canada.
Neuroscience. 1995 Nov;69(1):71-81. doi: 10.1016/0306-4522(95)00185-l.
The limbic system-associated membrane protein is a 64,000-68,000 mol.wt molecule known to be preferentially expressed by neurons in limbic structures of rats and cats. The present immunohistochemical study describes the distribution of this protein in the basal ganglia of Macaca fascicularis. The ventral striatum of the cynomolgus monkey displays a very intense immunostaining, whereas the dorsal striatum is much more weakly stained, except for some small zones scattered in the caudate nucleus and, to a lesser extent, in the putamen. These protein-rich zones are in register with striosomes, as visualized on adjacent sections immunostained for calbindin. At pallidal levels, immunostaining for the protein is observed only in the subcommissural regions, at the ventromedial tip of the internal pallidum, and in the caudoventral portion of the external pallidum. At nigral levels, the immunostaining is highly heterogeneous with a marked decreasing rostrocaudal gradient. The staining is most intense in nigral regions that receive striatal inputs and are enriched with calbindin. Nigral sectors populated by dopaminergic neurons, as visualized on adjacent sections immunostained for tyrosine hydroxylase, are largely devoid of immunoreactivity. In contrast, the immunostaining is uniformly intense in the ventral tegmental area. This study provides the first neuroanatomical evidence for teh existence of the limbic system-associated membrane protein in primate brain. It reveals that this glycoprotein is distributed in a highly heterogeneous manner in primate basal ganglia, where it preferentially labels regions that are anatomically and functionally linked to the limbic system.
边缘系统相关膜蛋白是一种分子量为64,000 - 68,000的分子，已知在大鼠和猫的边缘结构神经元中优先表达。本免疫组织化学研究描述了该蛋白在食蟹猴基底神经节中的分布。食蟹猴的腹侧纹状体显示出非常强烈的免疫染色，而背侧纹状体染色则弱得多，除了尾状核中散布的一些小区域以及在较小程度上的壳核。这些富含蛋白质的区域与纹状体小体相对应，这在相邻切片上用钙结合蛋白免疫染色时可以看到。在苍白球水平，该蛋白的免疫染色仅在连合下区域、内侧苍白球腹侧尖端以及外侧苍白球的尾腹部分观察到。在黑质水平，免疫染色高度异质，具有明显的从吻侧到尾侧的梯度降低。染色在接受纹状体输入且富含钙结合蛋白的黑质区域最为强烈。在相邻切片上用酪氨酸羟化酶免疫染色显示的由多巴胺能神经元组成的黑质区域基本上没有免疫反应性。相反，腹侧被盖区的免疫染色均匀强烈。这项研究为灵长类动物大脑中存在边缘系统相关膜蛋白提供了首个神经解剖学证据。它表明这种糖蛋白在灵长类动物基底神经节中以高度异质的方式分布，在那里它优先标记与边缘系统在解剖学和功能上相关的区域。
