Buznikov G A, Mal'chenko L A, Zvezdina N D, Iovanovic S, Markova L N, Milosevic I, Nikitina L A, Lazarevic L, Redzic L, Rogac L, Rakic L
Ontogenez. 1996 Jan-Feb;27(1):62-9.
The effects of the antitumor drug tiazofurin on development of sea urchins Sphaerechinus granularis, Paracentrotus lividus, Strongylocentrotus intermedius, and Arbacia lixula were studied. When 0.01-200 microM tiazofurin (TAF) was introduced in the incubation medium (artificial sea water) just after fertilization or at the midblastula stage, the development proceeded quite normally until the beginning of gastrulation. But later TAF blocked gastrulation and induced formation of mobile ball-shaped larvae with normal pigment cells but devoid of the nervous system, skeletal spicules and digestive tract. The threshold TAF concentrations varied from 0.05 microM (S. granularis) to 2-5 microM (all other species). When TAF was introduced during gastrulation and just after gastrulation, the larvae had defective nervous system and skeleton and suppressed expression of gangliosides. The nonhydrolyzable analog of GTP, GTP-gamma-S (5-20 microM), introduced in artificial sea water no later than at the midblastula stage prevented all above mentioned developmental defects.
研究了抗肿瘤药物噻唑呋林对球海胆、地中海海胆、中间球海胆和毒刺海胆发育的影响。在受精后或囊胚中期将0.01 - 200微摩尔的噻唑呋林（TAF）加入到孵化培养基（人工海水）中时，发育在原肠胚形成开始前都相当正常。但之后TAF会阻断原肠胚形成，并诱导形成具有正常色素细胞但没有神经系统、骨骼棘和消化道的可移动球形幼虫。TAF的阈值浓度从0.05微摩尔（球海胆）到2 - 5微摩尔（所有其他物种）不等。当在原肠胚形成期间及原肠胚形成后立即加入TAF时，幼虫的神经系统和骨骼有缺陷，神经节苷脂的表达受到抑制。在人工海水中不迟于囊胚中期加入不可水解的GTP类似物GTP-γ-S（5 - 20微摩尔）可防止上述所有发育缺陷。
