Murkovic M, Hillebrand A, Winkler J, Pfannhauser W
Graz University of Technology, Department of Bio- and Food Chemistry, Austria.
Z Lebensm Unters Forsch. 1996 Apr;202(4):275-8. doi: 10.1007/BF01206096.
Pumpkin (Cucurbita pepo L.) seed oil is a common salad oil which is produced in the southern parts of Austria, Slovenia and Hungary. It is dark green and has a high content of free fatty acids. Due to its colour, the oil cannot be used for cooking. The content of vitamin E, especially gamma-tocopherol, is very high. The oil content of the pumpkin seed is about 50%. The seed itself can be eaten. Therefore a pumpkin variety with high vitamin E content is desirable. The aim of this work was to find a variety of Cucurbita pepo which has a high oil yield and a high vitamin E content. A total of 100 breeding lines were tested for their tocopherol content. The tocopherols and tocotrienols are extracted with hexane and analysed by NP-HPLC/FLD with hexane/dioxan (96/4) as eluent, with fluorescence detection at 292/335 nm. The gamma-tocopherol content, which is about 5-10 times as much as that of alpha-tocopherol varies over a broad range (41-620 mg/kg dry pumpkin seeds). Beta- and delta-tocopherol are found at low levels.
南瓜（西葫芦）籽油是一种常见的色拉油，产于奥地利南部、斯洛文尼亚和匈牙利。它呈深绿色，游离脂肪酸含量高。由于其颜色，该油不能用于烹饪。维生素E的含量，尤其是γ-生育酚，非常高。南瓜籽的含油量约为50%。种子本身可以食用。因此，需要一种维生素E含量高的南瓜品种。这项工作的目的是找到一种西葫芦品种，其具有高油产量和高维生素E含量。总共对100个育种系的生育酚含量进行了测试。生育酚和生育三烯酚用己烷提取，并用正相高效液相色谱/荧光检测器（NP-HPLC/FLD）进行分析，以己烷/二恶烷（96/4）作为洗脱剂，在292/335 nm处进行荧光检测。γ-生育酚的含量约为α-生育酚的5至10倍，其变化范围很广（41-620毫克/千克干南瓜籽）。β-和δ-生育酚的含量较低。
