Bhambhani Y, Burnham R, Snydmiller G, MacLean I, Martin T
University of Alberta, Faculty of Rehabilitation Medicine, Edmonton, Canada.
Am Ind Hyg Assoc J. 1996 May;57(5):464-8. doi: 10.1080/15428119691014819.
This study compared the acute effects of 5 ppm hydrogen sulfide (H2S) inhalation (50 % of its occupational exposure limit) on the biochemical properties of skeletal muscle in exercising men and women. Twenty-five healthy volunteers, 13 men and 12 women, completed two 30-minute submaximal tests at 50% of their predetermined maximal aerobic power (VO2max) while breathing 0 ppm (control) or 5 ppm H2S from a specially designed flow system in a single-blind manner. Immediately after exercise, biopsies were obtained from the vastus lateralis muscle under local anaesthesia. They were subsequently analyzed for concentrations of the following markers of anaerobic and aerobic metabolism: lactate (La), lactate dehydrogenase (LDH), citrate synthase (CS), and cytochrome oxidase (CytOx). Repeated measures analysis of variance indicated that in men, the CS concentration decreased significantly (p = 0.006) as a result of H2S exposure. There was also a tendency for their La and LDH concentrations to increase and CytOx concentration to decrease in the presence of H2S, but these changes were not significant (p > 0.05). In women no significant changes were observed in any of these biochemical properties. These results suggest that (1) exposure to H2S at 50% of its OEL might inhibit aerobic metabolism during exercise in healthy men, thereby increasing their dependency on anaerobic metabolism; and (2) there could be a significant gender difference in the acute response to sub-OEL exposures of H2S.
本研究比较了吸入5 ppm硫化氢（H₂S）（其职业接触限值的50%）对正在进行运动的男性和女性骨骼肌生化特性的急性影响。25名健康志愿者，13名男性和12名女性，在以50%的预定最大有氧功率（VO₂max）完成两次30分钟的次最大测试时，以单盲方式通过专门设计的流动系统呼吸0 ppm（对照）或5 ppm H₂S。运动结束后立即在局部麻醉下从股外侧肌获取活检样本。随后对样本进行分析，检测以下无氧和有氧代谢标志物的浓度：乳酸（La）、乳酸脱氢酶（LDH）、柠檬酸合酶（CS）和细胞色素氧化酶（CytOx）。重复测量方差分析表明，在男性中，由于接触H₂S，CS浓度显著降低（p = 0.006）。在H₂S存在的情况下，他们的La和LDH浓度也有增加的趋势，CytOx浓度有降低的趋势，但这些变化不显著（p > 0.05）。在女性中，未观察到这些生化特性有任何显著变化。这些结果表明：（1）接触50%职业接触限值的H₂S可能会抑制健康男性运动期间的有氧代谢，从而增加他们对无氧代谢的依赖；（2）对于低于职业接触限值的H₂S暴露，急性反应可能存在显著的性别差异。
