Autieri M V, Prystowsky M B, Ohlstein E H
Department of Molecular Biology, Deborah Research Institute, Browns Mills, NJ 08015, USA.
DNA Cell Biol. 1996 Apr;15(4):297-304. doi: 10.1089/dna.1996.15.297.
In previous studies, differential display analysis of balloon angioplasty-damaged rat carotid arteries identified a temporally expressed partial cDNA termed RC9. This message is undetectable in undamaged vessels, reaches maximal levels 3 days post-procedure, and reduces to half-maximal expression by 14 days post angioplasty. Using RC9 to screen a cDNA library, we now report the isolation and characterization of a full-length clone, termed BART-1. BART-1 is 98% homologous to RC9 and shows the same mRNA expression pattern as RC9 in rat carotid arteries subject to balloon angioplasty. Northern analysis of various rat tissues reveals tissue specificity and possible differential processing. Neither the nucleic acid nor amino acid sequences demonstrate similarity to previously reported expressed sequences. Predicted amino acid analysis reveals two strongly hydrophilic and one hydrophobic region, suggesting a type II integral membrane protein. The cDNA sequence hybridized to genomic DNA from a variety of species, suggesting evolutionary conservation. Thus, BART-1 mRNA appears to represent an inducible, tissue-specific transcript encoding a putative integral membrane protein transiently expressed in response to vascular trauma.
在先前的研究中，对球囊血管成形术损伤的大鼠颈动脉进行差异显示分析，鉴定出一种在特定时间表达的部分cDNA，称为RC9。在未受损的血管中检测不到该信息，在手术后3天达到最高水平，在血管成形术后14天降至最大表达量的一半。利用RC9筛选cDNA文库，我们现在报告全长克隆BART-1的分离和鉴定。BART-1与RC9有98%的同源性，并且在接受球囊血管成形术的大鼠颈动脉中显示出与RC9相同的mRNA表达模式。对各种大鼠组织的Northern分析揭示了组织特异性和可能的差异加工。核酸和氨基酸序列均未显示与先前报道的表达序列相似。预测的氨基酸分析揭示了两个强亲水区和一个疏水区，表明这是一种II型整合膜蛋白。cDNA序列与来自多种物种的基因组DNA杂交，表明其具有进化保守性。因此，BART-1 mRNA似乎代表一种可诱导的、组织特异性的转录本，编码一种假定的整合膜蛋白，在血管损伤后短暂表达。
