Autieri M V
Department of Molecular Biology, Deborah Research Institute, Browns Mills, New Jersey 08015, USA.
Biochem Biophys Res Commun. 1996 Nov 1;228(1):29-37. doi: 10.1006/bbrc.1996.1612.
We have previously identified a partial cDNA fragment obtained from rat carotid arteries subject to balloon angioplasty which is increased in response to vascular injury. We now report the isolation and characterization of a full length human clone which is 98% homologous at the amino acid level to the rat. Allograft Inflammatory Factor-1 (AIF-1), a gene previously reported to be expressed in rat IFN gamma-activated macrophages. This transcript is expressed in low levels in undamaged, and increases 1 day and 3 days, and declines 7 days post balloon angioplasty. This human AIF-1 homologue is inducible in serum and cytokine-stimulated human smooth muscle cells. Interestingly, human tissue distribution indicates a constitutive expression in lymphoid tissue which can be augmented by mitogens. In summary, human AIF represents a cytokine inducible, tissue-specific, and highly conserved transcript transiently expressed in response to vascular trauma.
我们之前从接受球囊血管成形术的大鼠颈动脉中鉴定出一个部分cDNA片段，该片段会因血管损伤而增加。我们现在报告一个全长人类克隆的分离和特性，该克隆在氨基酸水平上与大鼠的同源性为98%。同种异体移植炎症因子-1（AIF-1），一个先前报道在大鼠IFNγ激活的巨噬细胞中表达的基因。该转录本在未受损时表达水平较低，在球囊血管成形术后1天和3天增加，7天后下降。这种人类AIF-1同源物在血清和细胞因子刺激的人类平滑肌细胞中可诱导表达。有趣的是，人类组织分布表明其在淋巴组织中组成性表达，有丝分裂原可增强这种表达。总之，人类AIF代表一种细胞因子诱导的、组织特异性的、高度保守的转录本，在血管创伤后短暂表达。
