Calvillo Y M, Alexander M
Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, N.Y. 14853, USA.
Appl Microbiol Biotechnol. 1996 Apr;45(3):383-90. doi: 10.1007/s002530050700.
A microbial consortium mineralized biphenyl sorbed to polyacrylic beads faster than the slow rate at which much of the compound was desorbed. Pure cultures of bacteria isolated from the consortium mineralized biphenyl in solution but not the sorbed compound. However, combinations of two strains did degrade biphenyl. The consortium did not reduce the surface tension in media containing sorbed biphenyl or biphenyl in solution, and addition of synthetic and microbially produced surfactants to pure cultures did not result in utilization of sorbed biphenyl by isolates able to use the soluble molecule. Cells from the consortium that were attached to continuously washed beads degraded the substrate. We suggest that bacteria may act on sorbed compounds without the necessity of an initial desorption and that the mechanism may involve cells attached to the particles rather than the excretion of a surfactant.
一个微生物群落矿化吸附在聚丙烯酸珠上的联苯的速度，比该化合物大部分被解吸的缓慢速度要快。从该群落中分离出的纯细菌培养物能矿化溶液中的联苯，但不能矿化吸附的化合物。然而，两种菌株的组合确实能降解联苯。该群落不会降低含有吸附联苯或溶液中联苯的培养基的表面张力，并且向纯培养物中添加合成和微生物产生的表面活性剂，并不会使能够利用可溶性分子的分离菌株利用吸附的联苯。附着在持续洗涤的珠子上的群落细胞降解了底物。我们认为，细菌可能无需初始解吸就能作用于吸附的化合物，并且该机制可能涉及附着在颗粒上的细胞，而非表面活性剂的分泌。
