Michael A J, Hofer J M, Ellis T H
Department of Genetics and Microbiology, Institute of Food Research, Norwich, UK.
Plant Mol Biol. 1996 Mar;30(5):1051-8. doi: 10.1007/BF00020815.
The C2H2 TFIIIA/Krüppel class of zinc finger proteins are an important group of regulatory nucleic acid binding factors and have been extensively studied in humans, Drosophila and yeast. We have employed 3' RACE PCR, using a highly degenerate oligonucleotide primer, for the facile isolation of a C2H2 zinc finger protein cDNA (Pszf1) from pea petals. The Pszf1 cDNA open reading frame potentially encodes a protein with two widely separated zinc fingers similar to zinc finger proteins from petunia and wheat. This class of two-fingered zinc finger proteins, possessing a wide and variable linker sequence, appears to be unique to plants. Three regions outside the zinc finger domains are also conserved between the members of the plant zinc finger protein family and one of these regions is a candidate nuclear localisation signal. The Pszf1 amino acid sequence is most similar to that of the petunia Epf1 protein, they possess an interfinger linker sequence of approximately the same length and they have a similar expression pattern with maximal transcript accumulation in mature petals, suggesting that Pszf1 may be the pea homologue of the petunia Epf1 zinc finger gene.
C2H2型锌指蛋白中的TFIIIA/Krüppel类是一类重要的调节性核酸结合因子，在人类、果蝇和酵母中已得到广泛研究。我们使用高度简并的寡核苷酸引物，通过3' RACE PCR，从豌豆花瓣中轻松分离出一种C2H2锌指蛋白cDNA（Pszf1）。Pszf1 cDNA开放阅读框可能编码一种含有两个相距较远的锌指的蛋白质，类似于矮牵牛和小麦中的锌指蛋白。这类具有广泛且可变连接序列的双指锌指蛋白似乎是植物所特有的。锌指结构域外的三个区域在植物锌指蛋白家族成员之间也保守，其中一个区域是候选核定位信号。Pszf1氨基酸序列与矮牵牛Epf1蛋白的氨基酸序列最相似，它们具有大致相同长度的指间连接序列，并且具有相似的表达模式，在成熟花瓣中转录本积累量最大，这表明Pszf1可能是矮牵牛Epf1锌指基因的豌豆同源物。
