Treisman J, Desplan C
Howard Hughes Medical Institute, Rockefeller University, New York 10021.
Nature. 1989 Sep 28;341(6240):335-7. doi: 10.1038/341335a0.
The first zygotic genes to be expressed during early Drosophila development are the gap genes. Their role is to read and interpret coarse positional information deposited in the egg by the mother and to refine it by cross-regulatory interactions and by controlling a class of pair-rule genes. Little is known about the molecular mechanisms by which the three cloned gap genes carry out their genetically defined functions. Here we report that the Krüppel (Kr) gene product (Kr) binds to the sequence AAGGGGTTAA, whereas the hunchback (hb) gene product (Hb) recognizes the consensus ACNCAAAAAANTA. We have identified binding sites for these proteins upstream of the two hb promoters, which we suggest could mediate the repression of hb by Kr and perhaps allow hb to influence its own expression.
在果蝇早期发育过程中最早表达的合子基因是间隙基因。它们的作用是读取和解释由母体沉积在卵子中的粗略位置信息，并通过交叉调节相互作用以及控制一类成对规则基因来对其进行细化。对于三个已克隆的间隙基因执行其遗传学定义功能的分子机制，我们了解得还很少。在此我们报告，克虏伯（Kr）基因产物（Kr）与序列AAGGGGTTAA结合，而驼背（hb）基因产物（Hb）识别共有序列ACNCAAAAAANTA。我们在两个hb启动子上游鉴定出了这些蛋白质的结合位点，我们认为这些位点可能介导Kr对hb的抑制作用，并且或许还能使hb影响其自身的表达。
