Kumar R M, Khuranna A
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, U.A.E. University, Al Ain, UAE.
J Obstet Gynaecol (Tokyo 1995). 1995 Dec;21(6):601-7. doi: 10.1111/j.1447-0756.1995.tb00919.x.
Investigation of human oocyte chromosomes that fail to fertilize may provide data essential to the assessment of reproductive failure. In view of this, 121 uncleaved human oocytes were analyzed cytogenetically to assess the type and frequency of chromosomal abnormality.
Oocyte recovery was done from patients undergoing in vitro fertilization (IVE) procedures. Then, these oocytes were preincubated and inseminated in vitro. Karyotyping was attempted in 121 oocytes lacking signs fertilization 50 h after insemination.
Sixty-nine oocytes were adequately karyotyped. The overall frequency of chromosomal aberration was 47.6%. Amongst these, 34.8% were aneuploidy, the frequency of which was significantly higher (p < 0.05) in patients > 35 years of age. Diploidy and hyperploidy was noted in 7.2% and 2.8% respectively. Translocation were noted in 2.8% and in 18.8% of human oocytes sperm chromosome condensation appeared prematurely in the G1 phase. No correlation was a found between specific chromosomal aberrations and type of fertility, stimulation treatment or gonadotropin levels.
The high rate of chromosomal disorders (47.6%) in human oocytes may contribute significantly towards their failure to fertilize in vitro.
对未受精的人类卵母细胞进行研究，可为评估生殖失败提供重要数据。鉴于此，对121个未分裂的人类卵母细胞进行了细胞遗传学分析，以评估染色体异常的类型和频率。
从接受体外受精（IVE）手术的患者中回收卵母细胞。然后，将这些卵母细胞进行预孵育并体外授精。在授精50小时后，对121个未出现受精迹象的卵母细胞进行核型分析。
69个卵母细胞获得了充分的核型分析结果。染色体畸变的总体频率为47.6%。其中，34.8%为非整倍体，在35岁以上的患者中，其频率显著更高（p < 0.05）。二倍体和多倍体分别占7.2%和2.8%。发现2.8%的人类卵母细胞存在易位，18.8%的人类卵母细胞中精子染色体在G1期过早凝聚。未发现特定染色体畸变与生育类型、刺激治疗或促性腺激素水平之间存在相关性。
人类卵母细胞中染色体异常的高发生率（47.6%）可能是其体外受精失败的重要原因。
