Pabai F, Kermasha S, Morin A
Department of Food Science and Agricultural Chemistry, McGill University, Ste-Anne-de-Bellevue, PQ, Canada.
Can J Microbiol. 1996 May;42(5):446-52. doi: 10.1139/m96-061.
The continuous cultivation technique was used to investigate the screening for lipase-producing microorganisms from four commercial starters suitable for the degradation of domestic wastes. Using this technique, three strains of lipase-producing bacteria were isolated and identified: Pantoea agglomerans (BB96CC1, BB168CC2) and Pseudomonas fluorescens (BW96CC1). In addition, butter fat induced more lipase production when present in the growth medium. Interesterification of butter fat triacylglycerols by enzymatic extracts of the isolated strains of microorganisms resulted in an appreciable interesterification yield, implying that hydrolysis was suppressed and interesterification of butter fat triacylglycerols was maximized in a microemulsion free-cosurfactant system.
采用连续培养技术，从四种适用于降解生活垃圾的商业发酵剂中筛选产脂肪酶的微生物。利用该技术，分离并鉴定出三株产脂肪酶细菌：成团泛菌（BB96CC1、BB168CC2）和荧光假单胞菌（BW96CC1）。此外，生长培养基中存在乳脂肪时可诱导产生更多脂肪酶。分离出的微生物菌株的酶提取物对乳脂肪三酰甘油进行酯交换反应，得到了可观的酯交换产率，这意味着在无微乳液辅助表面活性剂体系中，乳脂肪三酰甘油的水解受到抑制，酯交换反应达到最大化。
