Reid L H, Crider-Miller S J, West A, Lee M H, Massagué J, Weissman B E
Department of Patholoy and Laboratory Medicine, University of North Carolina, Chapel Hill 27599, USA.
Cancer Res. 1996 Mar 15;56(6):1214-8.
The p57KIP2 gene encodes an inhibitor of cyclin-dependent kinase activity, which negatively regulates cell cycle progression. The human p57 gene is located in 11p15.5, a region of DNA frequently altered in neoplasia. We have isolated a human genomic clone and mapped the p57 gene to a 2.2-kb region between D11S648 and D11S679. Sequence analysis revealed that the coding DNA of the human p57 gene is divided by 0.5-kb intron. A second intron was detected in the 3' untranslated region, indicating that the human p57 gene contains at least three exons. Our previous work with somatic cell hybrids mapped a tumor suppressor gene for the G401 Wilms' tumor cell line to a approximately 500-kb region of 11p15.5 that includes p57. Northern blot analysis detected a 0.8-kb p57 transcript in several of the G401 hybrid lines. However, p57 expression did not correlate with tumor suppression. These results suggest that p57 is not responsible for the tumor suppression observed in our somatic cell hybrid assay.
p57KIP2基因编码一种细胞周期蛋白依赖性激酶活性抑制剂，该抑制剂对细胞周期进程起负调控作用。人类p57基因位于11p15.5，这是一个在肿瘤形成过程中DNA常发生改变的区域。我们分离出了一个人类基因组克隆，并将p57基因定位到D11S648和D11S679之间的一个2.2kb区域。序列分析显示，人类p57基因的编码DNA被一个0.5kb的内含子隔开。在3'非翻译区检测到第二个内含子，这表明人类p57基因至少包含三个外显子。我们之前对体细胞杂种的研究将G401肾母细胞瘤细胞系的一个肿瘤抑制基因定位到11p15.5的一个约500kb区域，该区域包括p57。Northern印迹分析在几个G401杂种细胞系中检测到一个0.8kb的p57转录本。然而，p57的表达与肿瘤抑制并不相关。这些结果表明，p57并非我们的体细胞杂种试验中所观察到的肿瘤抑制的原因。
