Chaudhary A K, Reddy G R, Blair I A, Marnett L J
A.B. Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Carcinogenesis. 1996 May;17(5):1167-70. doi: 10.1093/carcin/17.5.1167.
Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct. M1G-dR has been detected in rat and human liver. Random mutagenesis studies with MDA-modified DNA and recent 32P-postlabeling studies indicate that in addition to M1G-dR, adducts to deoxyadenosine may also be formed. We have utilized liquid chromatography coupled with electrospray ionization tandem mass spectrometry to characterize an N6-oxopropenyl-2'-deoxyadenosine adduct (M1A-dR) in calf DNA modified with MDA.
丙二醛（MDA）是脂质过氧化的产物，可导致细菌和哺乳动物细胞发生突变以及大鼠患癌。MDA在体外与脱氧核苷发生反应，MDA与脱氧鸟苷的单体加合物（M1G-dR）是主要加合物。已在大鼠和人类肝脏中检测到M1G-dR。对MDA修饰的DNA进行的随机诱变研究以及最近的32P后标记研究表明，除了M1G-dR之外，还可能形成脱氧腺苷的加合物。我们利用液相色谱与电喷雾电离串联质谱联用技术对用MDA修饰的小牛DNA中的N6-氧代丙烯基-2'-脱氧腺苷加合物（M1A-dR）进行了表征。
