Buko V, Lukivskaya O, Nikitin V, Tarasov Y, Zavodnik L, Borodinsky A, Gorenshtein B, Janz B, Gundermann K J, Schumacher R
Department of Experimental Hepatology, Belarusian Academy of Sciences, Grodno, Belarus.
Cell Biochem Funct. 1996 Jun;14(2):131-7. doi: 10.1002/cbf.657.
Polyenoylphosphatidylcholine (PPC: 100 or 300 mg kg-1 b.w., by gastric intubation for 30 days) produced a clearcut protection of the liver of rats treated with alloxan (150 mg kg-1 b.w., i.p.). The liver of rats treated with alloxan was characterized by hydropic dystrophy and lymphocytic infiltrations. Treatment with alloxan increased serum gamma-GT and ALAT activities. The liver structure of rats treated with PPC did not differ from the liver of control animals. PPC normalized the biochemical abnormalities caused by the diabetes. The number of pancreatic islets and beta/alpha cell ratio decreased in the diabetic rats. A number of beta-cells in this group did not contain granules. PPC prevented the decrease in the number of islets and the beta/alpha cell ratio in the pancreas of the diabetic rats. The intensity of staining of beta-cell granules in the pancreas of PPC-treated rats had a position intermediate between the control and diabetic groups. Alloxan increased the blood glucose content where treatment with PPC decreased this. The results suggest that PPC acts as a cytoprotector in the liver and pancreas of rats with experimental diabetes induced by alloxan.
聚多烯磷脂酰胆碱（PPC：100或300毫克/千克体重，经胃插管给药30天）对用四氧嘧啶（150毫克/千克体重，腹腔注射）处理的大鼠肝脏产生了明显的保护作用。用四氧嘧啶处理的大鼠肝脏表现为水样变性和淋巴细胞浸润。四氧嘧啶处理使血清γ-谷氨酰转移酶（gamma-GT）和丙氨酸转氨酶（ALAT）活性升高。用PPC处理的大鼠肝脏结构与对照动物的肝脏无异。PPC使糖尿病引起的生化异常恢复正常。糖尿病大鼠的胰岛数量和β/α细胞比值降低。该组中的许多β细胞不含颗粒。PPC可防止糖尿病大鼠胰腺中胰岛数量和β/α细胞比值的减少。用PPC处理的大鼠胰腺中β细胞颗粒的染色强度介于对照组和糖尿病组之间。四氧嘧啶使血糖含量升高，而PPC处理可降低血糖含量。结果表明，PPC在由四氧嘧啶诱导的实验性糖尿病大鼠的肝脏和胰腺中起细胞保护作用。
