基于蛋白A基因多态性的耐甲氧西林金黄色葡萄球菌分子分型
Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism.
作者信息
Frénay H M, Bunschoten A E, Schouls L M, van Leeuwen W J, Vandenbroucke-Grauls C M, Verhoef J, Mooi F R
机构信息
Molecular Microbiology Unit, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
出版信息
Eur J Clin Microbiol Infect Dis. 1996 Jan;15(1):60-4. doi: 10.1007/BF01586186.
The polymorphic X-region of the protein A gene (spa) was used for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains. The X-region is characterized by a variable number (between 3 and 15) of small repeats. DNA sequencing of MRSA strains revealed 25 distinct repeats. Analysis of MRSA strains grown in vitro and in vivo revealed that the X-region was sufficiently stable for epidemiologic typing of MRSA strains. Spa typing of MRSA strains was compared to phage typing and, in general, concordance was found between the two methods. However, spa typing was more sensitive, allowing differentiation of strains within a particular phage type. Results obtained with spa typing suggest that hospital outbreaks may be caused by two or more MRSA strains. Spa typing may be an important tool in unravelling the spread of MRSA strains within and between hospitals.
蛋白A基因（spa）的多态性X区域用于耐甲氧西林金黄色葡萄球菌（MRSA）菌株的分子分型。X区域的特征是有可变数量（3至15个）的小重复序列。MRSA菌株的DNA测序揭示了25种不同的重复序列。对在体外和体内生长的MRSA菌株的分析表明，X区域对于MRSA菌株的流行病学分型足够稳定。将MRSA菌株的spa分型与噬菌体分型进行比较，总体上发现两种方法之间具有一致性。然而，spa分型更敏感，能够区分特定噬菌体类型内的菌株。spa分型获得的结果表明，医院暴发可能由两种或更多种MRSA菌株引起。Spa分型可能是揭示MRSA菌株在医院内部和医院之间传播的重要工具。
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