Gagerman E, Hellman B, Täljedal I B
Endocrinology. 1979 Apr;104(4):1000-2. doi: 10.1210/endo-104-4-1000.
Isolated pancreatic islets of noninbred ob/ob mice were used to test the hypothesis that adenylate cyclase responds to changes of the transmembrane milieu or electric field in intact beta-cells. In the presence of a phosphodiesterase inhibitor, ouabainstimulated both the release of insulin and the islet content of cAMP. Ouabain had no noticeable effect on the islet content of cGMP. These results support the hypothesis at test. However, because ouabain also had some stimulatory effect on cAMP in islet homogenates, a direct action of ouabain on adenylate cyclase cannot be ruled out.
使用非近交系ob/ob小鼠的分离胰岛来检验以下假设：腺苷酸环化酶对完整β细胞中跨膜环境或电场的变化作出反应。在磷酸二酯酶抑制剂存在的情况下，哇巴因刺激胰岛素释放和胰岛中cAMP的含量。哇巴因对胰岛中cGMP的含量没有明显影响。这些结果支持了所检验的假设。然而，由于哇巴因对胰岛匀浆中的cAMP也有一些刺激作用，因此不能排除哇巴因对腺苷酸环化酶的直接作用。
