利用抗肽抗体检测小鼠和仓鼠朊病毒蛋白（PrP）的物种特异性表位。

Detection of species specific epitopes of mouse and hamster prion proteins (PrPs) by anti-peptide antibodies.

作者信息

Yokoyama T, Itohara S, Yuasa N

机构信息

National Institute of Animal Health, Ibaraki, Japan.

出版信息

Arch Virol. 1996;141(3-4):763-9. doi: 10.1007/BF01718334.

DOI:10.1007/BF01718334

PMID:8645112

Abstract

Antisera to four synthetic peptides containing the substitutions between mouse and hamster prion proteins (PrPs) were produced in rabbits. The synthetic peptides used represent two mouse (Mo-I: residues 100-115 and Mo-V: residues 199-208) and two hamster PrP subregion sequences (Ha-I: 101-116 and Ha-V: 200-209). All antisera reacted strongly with homologous peptides but either not at all or poorly with heterologous peptides in enzymelinked immunosorbent assay (ELISA). Antisera to Mo-I and Mo-V recognized mouse PrPSc but not hamster PrpSc in western blot analysis (WB) and ELISA. Antisera to Ha-I contain antibodies specific to hamster PrPSc. The results indicate that these regions of PrPSc constitute species-specific epitopes. In contrast to these antisera, the antiserum to Ha-V recognized neither hamster nor mouse PrPSc. In this study, we identified mouse subregion-V as a species-specific epitope.

摘要

针对含有小鼠和仓鼠朊病毒蛋白（PrP）之间替换位点的四种合成肽，在兔体内制备了抗血清。所使用的合成肽代表两种小鼠PrP亚区域序列（Mo-I：第100 - 115位氨基酸残基和Mo-V：第199 - 208位氨基酸残基）以及两种仓鼠PrP亚区域序列（Ha-I：第101 - 116位氨基酸残基和Ha-V：第200 - 209位氨基酸残基）。在酶联免疫吸附测定（ELISA）中，所有抗血清与同源肽强烈反应，但与异源肽根本不反应或反应较弱。在蛋白质免疫印迹分析（WB）和ELISA中，针对Mo-I和Mo-V的抗血清能识别小鼠PrP^Sc，但不能识别仓鼠PrP^Sc。针对Ha-I的抗血清含有对仓鼠PrP^Sc特异的抗体。结果表明，PrP^Sc的这些区域构成种属特异性表位。与这些抗血清不同，针对Ha-V的抗血清既不能识别仓鼠PrP^Sc，也不能识别小鼠PrP^Sc。在本研究中，我们确定小鼠亚区域V为种属特异性表位。