通过核磁共振分析解析了盘基网柄菌中二磷酸 - 肌醇五磷酸和双二磷酸 - 肌醇四磷酸的结构。
Structures of diphospho-myo-inositol pentakisphosphate and bisdiphospho-myo-inositol tetrakisphosphate from Dictyostelium resolved by NMR analysis.
作者信息
Laussmann T, Eujen R, Weisshuhn C M, Thiel U, Vogel G
机构信息
Fachbereich 9-Chemie, Bergische Universität GHS Wuppertal, Federal Republic of Germany.
出版信息
Biochem J. 1996 May 1;315 ( Pt 3)(Pt 3):715-20. doi: 10.1042/bj3150715.
Abstract
Diphospho-myo-inositol phosphates (PP-InsP5 and bis-PP-InsP4) were isolated from Dictyostelium in order to clarify the precise positional isomerism by two-dimensional 1H/31P-NMR analysis. The diphosphorylated inositol phosphates are 4-PP-Ins(1,2,3,5,6)P5 and 4,5-bis-PP-Ins(1,2,3,6)P4 or their corresponding enantiomers. The vicinal arrangement of the diphospho groups with its steric and electrostatic constraints possibly qualifies bis-PP-InsP4 as a metabolite with high phosphate-group-transfer potential in phosphotransferase reactions.
摘要
为了通过二维1H/31P-NMR分析阐明精确的位置异构现象，从盘基网柄菌中分离出二磷酸-肌醇磷酸酯（PP-InsP5和双磷酸-PP-InsP4）。二磷酸化的肌醇磷酸酯为4-PP-Ins(1,2,3,5,6)P5和4,5-双磷酸-PP-Ins(1,2,3,6)P4或它们相应的对映体。二磷酸基团的邻位排列及其空间和静电限制可能使双磷酸-PP-InsP4成为磷酸转移酶反应中具有高磷酸基团转移潜力的代谢物。
相似文献
1
Structures of diphospho-myo-inositol pentakisphosphate and bisdiphospho-myo-inositol tetrakisphosphate from Dictyostelium resolved by NMR analysis.通过核磁共振分析解析了盘基网柄菌中二磷酸 - 肌醇五磷酸和双二磷酸 - 肌醇四磷酸的结构。
Biochem J. 1996 May 1;315 ( Pt 3)(Pt 3):715-20. doi: 10.1042/bj3150715.
2
Diphospho-myo-inositol phosphates from Dictyostelium identified as D-6-diphospho-myo-inositol pentakisphosphate and D-5,6-bisdiphospho-myo-inositol tetrakisphosphate.来自盘基网柄菌的二磷酸肌醇磷酸被鉴定为D-6-二磷酸肌醇五磷酸和D-5,6-双二磷酸肌醇四磷酸。
Biochem J. 1997 Feb 15;322 ( Pt 1)(Pt 1):31-3. doi: 10.1042/bj3220031.
3
Diphospho-myo-inositol phosphates in Dictyostelium and Polysphondylium: identification of a new bisdiphospho-myo-inositol tetrakisphosphate.盘基网柄菌和多孢网柄菌中的二磷酸 - 肌醇磷酸：一种新的双二磷酸 - 肌醇四磷酸的鉴定
FEBS Lett. 1998 Apr 10;426(1):145-50. doi: 10.1016/s0014-5793(98)00329-9.
4
neo-inositol polyphosphates in the amoeba Entamoeba histolytica.溶组织内阿米巴中新型肌醇多磷酸盐
J Biol Chem. 2000 Apr 7;275(14):10134-40. doi: 10.1074/jbc.275.14.10134.
5
myo-inositol pentakisphosphates. Structure, biological occurrence and phosphorylation to myo-inositol hexakisphosphate.肌醇五磷酸。结构、生物学存在形式以及磷酸化生成肌醇六磷酸。
Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):485-99. doi: 10.1042/bj2750485.
6
Product-precursor relationships amongst inositol polyphosphates. Incorporation of [32P]Pi into myo-inositol 1,3,4,6-tetrakisphosphate, myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 3,4,5,6-tetrakisphosphate and myo-inositol 1,3,4,5,6-pentakisphosphate in intact avian erythrocytes.肌醇多磷酸之间的产物-前体关系。[32P]磷酸根离子掺入完整禽红细胞中的肌醇1,3,4,6-四磷酸、肌醇1,3,4,5-四磷酸、肌醇3,4,5,6-四磷酸和肌醇1,3,4,5,6-五磷酸。
Biochem J. 1990 Jan 15;265(2):435-52. doi: 10.1042/bj2650435.
7
8
Enzyme-assisted total synthesis of the optical antipodes D-myo-inositol 3,4,5-trisphosphate and D-myo-inositol 1,5, 6-trisphosphate: aspects of their structure-activity relationship to biologically active inositol phosphates.光学对映体D-肌醇3,4,5-三磷酸酯和D-肌醇1,5,6-三磷酸酯的酶促全合成：它们与生物活性肌醇磷酸酯的构效关系方面
J Med Chem. 1999 Apr 8;42(7):1262-73. doi: 10.1021/jm981113k.
9
Conformational studies of myo-inositol phosphates.肌醇磷酸酯的构象研究。
Carbohydr Res. 1996 Dec 24;296:39-54. doi: 10.1016/s0008-6215(96)00250-9.
10
Identification and purification of diphosphoinositol pentakisphosphate kinase, which synthesizes the inositol pyrophosphate bis(diphospho)inositol tetrakisphosphate.二磷酸肌醇五磷酸激酶的鉴定与纯化，该激酶可合成焦磷酸肌醇双（二磷酸）肌醇四磷酸。
Biochemistry. 1998 Oct 20;37(42):14998-5004. doi: 10.1021/bi981920l.
引用本文的文献
1
Visualizing and Identifying Inositol Pyrophosphate Isomers in Crystallo.在晶体中可视化并鉴定肌醇焦磷酸异构体
Methods Mol Biol. 2025;2972:189-203. doi: 10.1007/978-1-0716-4799-8_14.
2
Absolute Configurations of Inositol Poly- and Pyrophosphates Assigned by Nuclear Magnetic Resonance Spectroscopy.通过核磁共振光谱法确定的肌醇多磷酸酯和焦磷酸酯的绝对构型
Methods Mol Biol. 2025;2972:115-123. doi: 10.1007/978-1-0716-4799-8_9.
3
Assigning the Absolute Configuration of Inositol Poly- and Pyrophosphates by NMR Using a Single Chiral Solvating Agent.利用手性溶剂通过 NMR 对肌醇多聚和焦磷酸盐的绝对构型进行赋值。
Biomolecules. 2023 Jul 19;13(7):1150. doi: 10.3390/biom13071150.
4
The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis.粘菌（Dictyostelium discoideum）的肌醇六磷酸焦磷酸代谢并不调控无机多聚磷酸盐（polyP）的合成。
Adv Biol Regul. 2022 Jan;83:100835. doi: 10.1016/j.jbior.2021.100835. Epub 2021 Nov 10.
5
Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry.采用毛细管电泳-电喷雾电离质谱法分析肌醇磷酸盐代谢。
Nat Commun. 2020 Nov 27;11(1):6035. doi: 10.1038/s41467-020-19928-x.
6
The Key Role of IPK: A Novel Target for Anticancer Treatments?IPK 的关键作用：抗癌治疗的新靶点？
Molecules. 2020 Sep 25;25(19):4401. doi: 10.3390/molecules25194401.
7
Harnessing C-labeled -inositol to interrogate inositol phosphate messengers by NMR.利用碳标记的肌醇通过核磁共振技术研究肌醇磷酸信使分子。
Chem Sci. 2019 Apr 23;10(20):5267-5274. doi: 10.1039/c9sc00151d. eCollection 2019 May 28.
8
Inositol Polyphosphate Kinases, Fungal Virulence and Drug Discovery.肌醇多磷酸激酶、真菌致病性与药物发现
J Fungi (Basel). 2016 Sep 6;2(3):24. doi: 10.3390/jof2030024.
9
The inositol pyrophosphate pathway in health and diseases.肌醇六磷酸途径在健康和疾病中的作用。
Biol Rev Camb Philos Soc. 2018 May;93(2):1203-1227. doi: 10.1111/brv.12392. Epub 2017 Dec 27.
10
The emerging roles of inositol pyrophosphates in eukaryotic cell physiology.肌醇焦磷酸在真核细胞生理学中的新作用。
J Biosci. 2015 Sep;40(3):593-605. doi: 10.1007/s12038-015-9549-x.
本文引用的文献
1
Application of two-dimensional total correlation spectroscopy for structure determination of individual inositol phosphates in a mixture.二维全相关光谱法在混合物中单个肌醇磷酸酯结构测定中的应用。
Anal Biochem. 1995 Nov 1;231(2):421-31. doi: 10.1006/abio.1995.0073.
2
3
Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells.胰腺癌细胞中肌醇多磷酸焦磷酸的周转
J Biol Chem. 1993 Feb 25;268(6):3850-6.
4
Turnover of inositol pentakisphosphates, inositol hexakisphosphate and diphosphoinositol polyphosphates in primary cultured hepatocytes.原代培养肝细胞中肌醇五磷酸、肌醇六磷酸和二磷酸肌醇多磷酸的周转
Biochem J. 1993 Jul 15;293 ( Pt 2)(Pt 2):583-90. doi: 10.1042/bj2930583.
5
31P-NMR analysis of Entamoeba histolytica. Occurrence of high amounts of two inositol phosphates.溶组织内阿米巴的31P-核磁共振分析。两种肌醇磷酸大量存在的情况。
Eur J Biochem. 1993 Jun 15;214(3):711-8. doi: 10.1111/j.1432-1033.1993.tb17972.x.
6
Golgi coatomer binds, and forms K(+)-selective channels gated by, inositol polyphosphates.高尔基体衣被蛋白结合肌醇多磷酸并形成由其门控的钾离子选择性通道。
J Biol Chem. 1994 Jul 8;269(27):17826-32.
7
8
Synthesis and metabolism of bis-diphosphoinositol tetrakisphosphate in vitro and in vivo.双二磷酸肌醇四磷酸在体外和体内的合成与代谢
J Biol Chem. 1995 May 5;270(18):10489-97. doi: 10.1074/jbc.270.18.10489.
9
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.细胞黏菌盘基网柄菌的黏变形体在无菌培养中的生长。
Biochem J. 1970 Sep;119(2):171-4. doi: 10.1042/bj1190171.
10
A novel metal-dye detection system permits picomolar-range h.p.l.c. analysis of inositol polyphosphates from non-radioactively labelled cell or tissue specimens.一种新型金属染料检测系统可对来自非放射性标记细胞或组织标本的肌醇多磷酸进行皮摩尔级别的高效液相色谱分析。
Biochem J. 1988 Sep 1;254(2):585-91. doi: 10.1042/bj2540585.
Zotero 插件