Zong Guangning, Wang Huanchen
Genomic Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
Molecular and Cellular Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
Methods Mol Biol. 2025;2972:189-203. doi: 10.1007/978-1-0716-4799-8_14.
Inositol pyrophosphates (PP-InsPs), which possess functionally significant diphosphate groups, regulate a wide range of biological processes across eukaryotic organisms. The presence and isomeric diversity of PP-InsPs suggest potential biological relevance and enable these molecules to serve as versatile regulators. Identification and characterization of the PP-InsP isomers require specialized approaches. We have successfully utilized X-ray crystallography to directly visualize and identify PP-InsP isomers, thereby informing biological studies and advancing our understanding of their functional roles. Here, we describe detailed methods for identifying PP-InsP isomers in Crystallo.
肌醇焦磷酸（PP-InsPs）含有具有重要功能的二磷酸基团，可调节真核生物中的多种生物过程。PP-InsPs的存在及其异构体多样性表明其具有潜在的生物学相关性，并使这些分子能够作为多功能调节剂发挥作用。PP-InsP异构体的鉴定和表征需要专门的方法。我们已成功利用X射线晶体学直接可视化和鉴定PP-InsP异构体，从而为生物学研究提供信息并增进我们对其功能作用的理解。在此，我们描述了在晶体中鉴定PP-InsP异构体的详细方法。
