Hidaka H, Oshima T, Ikeda K, Furukawa M, Takasaka T
Department of Otorhinolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Biochem Biophys Res Commun. 1996 Mar 18;220(2):425-30. doi: 10.1006/bbrc.1996.0421.
Nonsensory epithelial cells of the mammalian cochlea contribute to the production of the endolymph. The presence as well as the function of the Na-K-Cl cotransporter has been suggested, but not yet been proved. To identify the Na-K-Cl cotransporter isoforms expressed in the cochlea, mRNA was extracted from the cochlear lateral wall. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR) with primers specific for Na-K-Cl cotransporter isoforms. In the rat cochlear lateral wall, mRNA homologous to the mouse bumetanide-sensitive Na-K-Cl cotransporter (mBSC2) was detected. These results suggest that the basolateral localization of the cotransporter in the marginal cell is involved in the secretory process of the endolymph.
哺乳动物耳蜗的非感觉上皮细胞有助于内淋巴的产生。有人提出了钠-钾-氯协同转运蛋白的存在及其功能，但尚未得到证实。为了鉴定耳蜗中表达的钠-钾-氯协同转运蛋白亚型，从耳蜗外侧壁提取mRNA。反转录后，用针对钠-钾-氯协同转运蛋白亚型的引物通过聚合酶链反应（PCR）扩增所得的cDNA。在大鼠耳蜗外侧壁中，检测到与小鼠布美他尼敏感钠-钾-氯协同转运蛋白（mBSC2）同源的mRNA。这些结果表明，协同转运蛋白在边缘细胞的基底外侧定位参与了内淋巴的分泌过程。
