Oshima T, Ikeda K, Furukawa M, Takasaka T
Department of Otolaryngology, Tohoku University School of Medicine 1-1, Sendai, Japan.
Biochem Biophys Res Commun. 1997 Apr 28;233(3):737-41. doi: 10.1006/bbrc.1997.6533.
The cochlea has been suggested to express some Na+/ Ca2+ exchangers (NCX), since efficient acoustic transduction requires cytosolic calcium homeostasis. The present study revealed that several spliced isoforms of NCX are expressed in the guinea pig cochlea. Moreover, to determine their localization in the cochlea, microdissected RT-PCR was performed. The guinea pig cochlea was microdissected into three parts (lateral wall, the organ of Corti and modiolus). The cochlear lateral wall and the organ of Corti expressed only a single isoform of NCX1. On the other hand, five isoforms of NCX1 and four isoforms of NCX3 were detected in the cochlear modiolus. The alternative splicing may provide diverse functions for NCX in the cochlea.
由于有效的听觉转导需要胞质钙稳态，因此有人提出耳蜗表达一些钠/钙交换体（NCX）。本研究表明，NCX的几种剪接异构体在豚鼠耳蜗中表达。此外，为了确定它们在耳蜗中的定位，进行了显微切割RT-PCR。将豚鼠耳蜗显微切割成三个部分（外侧壁、柯蒂氏器和蜗轴）。耳蜗外侧壁和柯蒂氏器仅表达NCX1的单一异构体。另一方面，在蜗轴中检测到NCX1的五种异构体和NCX3的四种异构体。可变剪接可能为耳蜗中的NCX提供多种功能。
