Lin T J, Zhang K J, Liu G T
Institute of Matera Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P.R. China.
Biochem Pharmacol. 1996 May 3;51(9):1237-41. doi: 10.1016/0006-2952(96)00067-6.
Salvianolic acid A (Sai A) has demonstrated potent antioxidant activity in previous studies. In the present study, the effects of Sai A on oxygen radicals released by rat neutrophils and on neutrophil function were investigated. Superoxide anion assayed by the nitroblue tetrazolium test and hydrogen peroxide detected with redox of scopoletin were scavenged concentration dependently by Sai A in n-formyl-methionyl-leucylphenylalanine (fMLP)- and phorbol myristate acetate (PMA)-stimulated rat neutrophils. Hydroxyl radicals generated in PMA-stimulated neutrophils, measured by HPLC, also were scavenged significantly by Sai A, whereas Sai A showed no significant effects on chemotaxis toward fMLP and phagocytosis of latex beads by rat neutrophils. In addition, the intracellular free calcium and cyclic nucleotide levels of neutrophils, when stimulated by fMLP, were not affected by Sai A. These results suggest that Sai A could significantly scavenge oxygen radicals released by activated neutrophils without affecting their functional ability.
丹酚酸A（Salvianolic acid A，Sai A）在先前的研究中已显示出强大的抗氧化活性。在本研究中，研究了Sai A对大鼠中性粒细胞释放的氧自由基以及中性粒细胞功能的影响。在经N-甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）和佛波酯（PMA）刺激的大鼠中性粒细胞中，Sai A以浓度依赖的方式清除了通过氮蓝四唑试验测定的超氧阴离子以及通过 scopoletin氧化还原检测到的过氧化氢。通过高效液相色谱法测量，在PMA刺激的中性粒细胞中产生的羟基自由基也被Sai A显著清除，而Sai A对大鼠中性粒细胞向fMLP的趋化作用和乳胶珠的吞噬作用没有显著影响。此外，当受到fMLP刺激时，中性粒细胞的细胞内游离钙和环核苷酸水平不受Sai A的影响。这些结果表明，Sai A可以显著清除活化中性粒细胞释放的氧自由基，而不影响其功能能力。
