Effects of salvianolic acid A on oxygen radicals released by rat neutrophils and on neutrophil function.

Salvianolic acid A (Sai A) has demonstrated potent antioxidant activity in previous studies. In the present study, the effects of Sai A on oxygen radicals released by rat neutrophils and on neutrophil function were investigated. Superoxide anion assayed by the nitroblue tetrazolium test and hydrogen peroxide detected with redox of scopoletin were scavenged concentration dependently by Sai A in n-formyl-methionyl-leucylphenylalanine (fMLP)- and phorbol myristate acetate (PMA)-stimulated rat neutrophils. Hydroxyl radicals generated in PMA-stimulated neutrophils, measured by HPLC, also were scavenged significantly by Sai A, whereas Sai A showed no significant effects on chemotaxis toward fMLP and phagocytosis of latex beads by rat neutrophils. In addition, the intracellular free calcium and cyclic nucleotide levels of neutrophils, when stimulated by fMLP, were not affected by Sai A. These results suggest that Sai A could significantly scavenge oxygen radicals released by activated neutrophils without affecting their functional ability.