Takahashi A, Taniguchi T, Fujioka Y, Ishikawa Y, Yokoyama M
First Department of Internal Medicine, Kobe University School of Medicine, Japan.
Atherosclerosis. 1996 Feb;120(1-2):93-9. doi: 10.1016/0021-9150(95)05686-6.
We investigated the effects of lipoprotein(a) (Lp(a)) and low density lipoprotein (LDL) on proliferation of human umbilical vein endothelial cells (HUVECs). Both Lp(a) and LDL stimulated the growth of HUVECs synergistically with basic fibroblast growth factor and insulin in a dose-dependent manner. The potency of Lp(a) to promote the cell proliferation was 40% less than that of LDL. Addition of anti-transforming growth factor-beta 1 neutralizing antibody into the medium could not diminish the difference of HUVECs proliferation by Lp(a) and LDL. However, addition of anti-LDL receptor antibody suppressed HUVECs proliferation to the same level and sequestered the difference by the two lipoproteins. Moreover, cholesteryl ester content incubated with Lp(a) was 50% less than that with LDL. These results suggest that Lp(a) has less effect on HUVECs proliferation and cholesterol delivery to the cells than LDL. Therefore, Lp(a) may play a role as an atherogenic lipoprotein by delaying the repair of endothelium after injury.
我们研究了脂蛋白(a) [Lp(a)]和低密度脂蛋白(LDL)对人脐静脉内皮细胞(HUVECs)增殖的影响。Lp(a)和LDL均能与碱性成纤维细胞生长因子和胰岛素协同，以剂量依赖的方式刺激HUVECs的生长。Lp(a)促进细胞增殖的能力比LDL低40%。向培养基中添加抗转化生长因子-β1中和抗体并不能消除Lp(a)和LDL对HUVECs增殖的差异。然而，添加抗LDL受体抗体可将HUVECs增殖抑制到相同水平，并消除两种脂蛋白之间的差异。此外，与Lp(a)一起孵育的胆固醇酯含量比与LDL一起孵育的少50%。这些结果表明，Lp(a)对HUVECs增殖和胆固醇向细胞内转运的影响比LDL小。因此，Lp(a)可能通过延迟损伤后内皮的修复而发挥致动脉粥样硬化脂蛋白的作用。
