Aïssa J, Harran H, Rabeau M, Boucherie S, Brouilhet H, Benveniste J
INSERM U 200, Clamart, France.
Int Arch Allergy Immunol. 1996 Jun;110(2):182-6. doi: 10.1159/000237285.
The tissue concentrations of several inflammatory mediators were determined from day 0 to day 60 in granuloma induced in rats (n = 105) by injection of carrageenan in the fascia of the latissimus dorsi muscle. Noncollagen proteins (NCP) and the number of polymorphonuclear leukocytes (PMN) and mast cells were also assessed. In comparison with the tissue at time 0, we noted in the inflamed tissue (at 4 h) an increase in total proteins (4.0 +/- 3.0 vs. 84 +/- 12.0%, mean +/- SEM) and PMN (0.0 +/- 0.0 vs. 43.3 +/- 13.4%), and a fall in histamine concentration (from 30.0 +/- 9.0 to 9.0 +/- 4.0 ng/ml). A partial disappearance of mast cells and an increase of PAF-acether (PAF) levels (1.0 +/- 1.0 vs. 30.0 +/- 22.0 ng/ml) was noted at 16 h, whereas lysopaf remained unchanged (3.7 +/- 4.0 vs. 3.5 +/- 1.0 ng/ml). During evolution towards chronic inflammation (day 10-60), NCP decreased, PMN disappeared and mast cells reappeared; the histamine level rose to 11.0 +/- 2.0 mg/ml, thus not reaching back baseline values. Lysopaf rose to 7.1 +/- 12.2 ng/ml and PAF levels increased further to reach 240.0 +/- 153.0 ng/ml at day 10. This study suggests that PAF may contribute to the acute phase of an inflammatory state such as the carrageenan-induced granuloma and that it is also present during the chronic process.
通过在大鼠（n = 105）背阔肌筋膜内注射角叉菜胶诱导肉芽肿，测定了从第0天到第60天几种炎症介质的组织浓度。还评估了非胶原蛋白（NCP）、多形核白细胞（PMN）数量和肥大细胞数量。与0时的组织相比，我们注意到在炎症组织（4小时时）中总蛋白增加（4.0±3.0 vs. 84±12.0%，平均值±标准误）和PMN增加（0.0±0.0 vs. 43.3±13.4%），组胺浓度下降（从30.0±9.0降至9.0±4.0 ng/ml）。在16小时时，观察到肥大细胞部分消失且血小板活化因子（PAF）水平增加（1.0±1.0 vs. 30.0±22.0 ng/ml），而溶血PAF保持不变（3.7±4.0 vs. 3.5±1.0 ng/ml）。在向慢性炎症发展的过程中（第10 - 60天），NCP减少，PMN消失且肥大细胞重新出现；组胺水平升至11.0±2.0 mg/ml，因此未恢复到基线值。溶血PAF升至7.1±12.2 ng/ml，PAF水平在第10天进一步增加至240.0±153.0 ng/ml。本研究表明，PAF可能参与了炎症状态的急性期，如角叉菜胶诱导的肉芽肿，并且在慢性过程中也存在。
