Jacquot J, Maizières M, Spilmont C, Millot J M, Sébille S, Merten M, Kammouni W, Manfait M
INSERM U. 314, Université de Reims, France.
FEBS Lett. 1996 May 20;386(2-3):123-7. doi: 10.1016/0014-5793(96)00405-x.
This study documents a difference between cystic fibrosis human (CF-HTG) and normal human (HTG) tracheal gland cells: the ability of histamine to induce an increase of intracellular free calcium concentration [Ca2+]i was abnormally reduced in CF-HTG cells. The magnitude of the [Ca2+]i peak rise in response to histamine is smaller in CF-HTG cells than in HTG cells, and the percentage of CF-HTG cells that increase [Ca2+]i is decreased compared with HTG cells. In contrast to histamine, the human neutrophil elastase (HNE) stimulation of both CF-HTG and HTG cells generated [Ca2+]i asynchronous oscillations and the magnitude of the peak [Ca2+]i response as well as the percentage of responding cells were similar for both groups. By videomicroscopy observations, the secretory response (exocytosis of secretion granules) of CF-HTG cells occurred with HNE, but not with histamine, thus suggesting that [Ca2+]i asynchronous oscillations may be linked to the exocytosis process in human tracheal gland cells.
本研究记录了囊性纤维化患者的人气管腺细胞（CF-HTG）与正常人气管腺细胞（HTG）之间的差异：组胺诱导细胞内游离钙浓度[Ca2+]i升高的能力在CF-HTG细胞中异常降低。与HTG细胞相比，CF-HTG细胞中组胺刺激引起的[Ca2+]i峰值升高幅度较小，且[Ca2+]i升高的CF-HTG细胞百分比降低。与组胺不同，人中性粒细胞弹性蛋白酶（HNE）刺激CF-HTG和HTG细胞均产生[Ca2+]i异步振荡，两组的[Ca2+]i峰值反应幅度以及反应细胞百分比相似。通过视频显微镜观察，CF-HTG细胞的分泌反应（分泌颗粒的胞吐作用）由HNE引发，但组胺不能引发，这表明[Ca2+]i异步振荡可能与人气管腺细胞的胞吐过程有关。
