Laterveer F D, Nicolay K, Gellerich F N
Department of in vivo NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.
FEBS Lett. 1996 May 20;386(2-3):255-9. doi: 10.1016/0014-5793(96)00455-3.
Macromolecules were added to isolated rat liver mitochondria to mimic cytosolic macromolecules and tested for their effects on the ADP delivery from adenylate kinase in the intermembrane space to oxidative phosphorylation. In the presence of 10% (w/v) dextran M20 or bovine serum albumin, approximately 60% of the maximal ADP flux from adenylate kinase to oxidative phosphorylation was not accessible to an extramitochondrial ADP scavenger. In the absence of macromolecules this was 34%. ADP determinations from incubations with macromolecules demonstrated the existence of flux-dependent ADP concentration gradients across the outer membrane which can be as high as 12 microM.
将大分子添加到分离的大鼠肝脏线粒体中，以模拟胞质大分子，并测试它们对腺苷酸激酶从膜间隙向氧化磷酸化传递ADP的影响。在存在10%（w/v）葡聚糖M20或牛血清白蛋白的情况下，线粒体外ADP清除剂无法接触到约60%从腺苷酸激酶到氧化磷酸化的最大ADP通量。在不存在大分子的情况下，这一比例为34%。对含有大分子的孵育物进行的ADP测定表明，跨外膜存在通量依赖性的ADP浓度梯度，其可高达12微摩尔。
