Murphy S G, Laufman H, LoBuglio A F
J Natl Cancer Inst. 1977 Jun;58(6):1815-8. doi: 10.1093/jnci/58.6.1815.
Strain 2 guinea pigs were immunized with the LE-L2C cell line and challenged with either LE-L2C or BZ-L2C, a subline of L2C leukemia deficient in la gene product and C3 receptor. The LE-L2C-immune animals were completely protected from a challenge of 5 X 10(6) cells (100 times the lethal dose) of either cell line, and the delayed skin test responses for both lines of injected cells were equivalent. Thus established in vivo immune recognition of the leukemia antigen was similar for both cell lines. After repeated immunization of strain 2 animals with BZ-L2C cells, animals were challenged with 3 X 10(5) viable cells; 9 of 13 animals survived. A subsequent 5 x 10 (6) challenge lowered survival to 8 of the 13 animals. The skin test response to BZ-L2C developed slowly during the immunization period, but survivors of the viable cell challenge exhibited good responses to skin testing with BZ-L2C or LE-L2C leukemic cells. Thus the BZ-L2C cell line possesses a leukemia-specific antigen, but the immunogenicity of this mutant line is decreased when compared to that of the LE-L2C line.
用LE-L2C细胞系对2系豚鼠进行免疫，然后用LE-L2C或BZ-L2C进行攻击，BZ-L2C是L2C白血病的一个亚系，缺乏Ia基因产物和C3受体。用LE-L2C免疫的动物对两种细胞系5×10⁶个细胞（致死剂量的100倍）的攻击具有完全的抵抗力，并且对两种注射细胞系的迟发型皮肤试验反应相当。因此，两种细胞系在体内对白血病抗原的免疫识别是相似的。在用BZ-L2C细胞对2系动物进行重复免疫后，用3×10⁵个活细胞对动物进行攻击；13只动物中有9只存活。随后用5×10⁶个细胞进行攻击，使13只动物中的存活数降至8只。在免疫期间，对BZ-L2C的皮肤试验反应发展缓慢，但活细胞攻击的存活者对用BZ-L2C或LE-L2C白血病细胞进行皮肤试验表现出良好的反应。因此，BZ-L2C细胞系具有白血病特异性抗原，但与LE-L2C系相比，该突变系的免疫原性降低。
