Mori O, Hachisuka H, Kiyokawa C, Sasai Y
Department of Dermatology, Kurume University School of Medicine, Japan.
J Dermatol. 1995 Dec;22(12):917-20. doi: 10.1111/j.1346-8138.1995.tb03945.x.
In the previous study, we demonstrated apoptotic cells in basal cell carcinomas by means of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL). Positive staining cells were present at the peripheries and centers of the nests. The number of positive cells increased in cases showing deep infiltration. We also stained other epidermal neoplasms using this modified TUNEL method. In squamous cell carcinomas, many positive cells were observed in poorly differentiated types, but only a few cells were stained in well-differentiated types. TUNEL positive cells were rare in Bowen's disease. No positive cells were seen in seborrheic keratosis or verruca vulgaris. Our studies showed that apoptotic cells were more common in rapid growth neoplasms than in slow growth neoplasms.
在先前的研究中，我们通过末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）在基底细胞癌中证实了凋亡细胞。阳性染色细胞出现在巢状结构的周边和中心。在表现为深部浸润的病例中，阳性细胞数量增加。我们还用这种改良的TUNEL方法对其他表皮肿瘤进行了染色。在鳞状细胞癌中，低分化型可见许多阳性细胞，而高分化型仅有少数细胞被染色。鲍温病中TUNEL阳性细胞很少见。脂溢性角化病或寻常疣中未见阳性细胞。我们的研究表明，凋亡细胞在快速生长的肿瘤中比在缓慢生长的肿瘤中更常见。
