Zhang H, German J B
Department of Food Science and Technology, University of California, Davis 95616, USA.
Lipids. 1996 Jan;31(1):19-25. doi: 10.1007/BF02522405.
This study investigated the potential of dietary fats to modulate the arachidonic acid content of mouse lung phospholipids and the formation of lipoxygenase products from arachidonic and eicosapentaenoic acids. Prior to breeding, female mice were fed for five months diets with 10 wt% of either olive oil, safflower oil, fish oil, or linseed oil. The same diets were fed to the females during gestation and to the pups from day 18 to day 42 postpartum. On day 42, the phospholipids were extracted from fresh lung tissue and separated into classes [phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylcholine (PC), and phosphatidylinositol (PI)] by thin-layer chromatography. Methyl esters of phospholipid fatty acids and unesterified fatty acids were analyzed by gas chromatography. At comparable dietary n-3/n-6 ratios, arachidonic acid was reduced 85 and 75% in lungs from mice fed linseed oil and fish oil, respectively, compared to lungs of safflower oil-fed mice. Dietary fats affected the proportion of arachidonic acid in phospholipids in the order: PE > PC > PS > PI. Following incubation of homogenized lung tissue, the total amount of 12-lipoxygenase products was lowest in lungs from mice fed olive oil, and 12-hydroxyeicosatetraenoic acid was lowest in incubated lungs from mice fed linseed oil. Comparison of the amounts of lipoxygenase substrate fatty acids in the individual phospholipids with the lipoxygenase products suggested that the major substrate pool for the 12-lipoxygenase pathway in mouse lung homogenates was PC.
本研究调查了膳食脂肪调节小鼠肺磷脂中花生四烯酸含量以及由花生四烯酸和二十碳五烯酸形成脂氧合酶产物的潜力。在繁殖前，给雌性小鼠喂食含10 wt%橄榄油、红花油、鱼油或亚麻籽油的饲料五个月。在妊娠期间给雌性小鼠喂食相同的饲料，并在产后第18天至第42天给幼崽喂食相同的饲料。在第42天，从新鲜肺组织中提取磷脂，并通过薄层色谱法将其分离为不同类别[磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）、磷脂酰胆碱（PC）和磷脂酰肌醇（PI）]。通过气相色谱法分析磷脂脂肪酸甲酯和未酯化脂肪酸。在可比的膳食n-3/n-6比例下，与喂食红花油的小鼠肺相比，喂食亚麻籽油和鱼油的小鼠肺中花生四烯酸分别减少了85%和75%。膳食脂肪对磷脂中花生四烯酸比例的影响顺序为：PE＞PC＞PS＞PI。在匀浆肺组织孵育后，喂食橄榄油的小鼠肺中12-脂氧合酶产物的总量最低，喂食亚麻籽油的小鼠孵育肺中12-羟基二十碳四烯酸最低。将各个磷脂中脂氧合酶底物脂肪酸的量与脂氧合酶产物进行比较表明，小鼠肺匀浆中12-脂氧合酶途径的主要底物库是PC。
