通过荧光显微镜检测组织培养中的支原体污染。
Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy.
作者信息
Thangavelu M, Ernø H, Therkelsen A J
出版信息
Hum Genet. 1979 Mar 12;47(2):199-202. doi: 10.1007/BF00273202.
PMID:86495
Abstract
The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher, i.e., at one mycoplasma or more per tissue-culture cell.
摘要
用精氨酸支原体对人皮肤成纤维细胞培养物进行可控污染后，对陈（1977年）检测组织培养中支原体污染物的原位染色方法进行了测试。得出的结论是，该方法仅在感染率为100%或更高时才可靠，即每个组织培养细胞有一个或多个支原体时才可靠。
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