Cavrois M, Gessain A, Wain-Hobson S, Wattel E
INSERM Institut de Recherche sur le Cancer de Lille, France.
Oncogene. 1996 Jun 6;12(11):2419-23.
Assuming that the clonal expansion of T cells harbouring the human T-cell leukemia virus type 1 (HTLV-1) provirus is a central feature of HTLV-1 infection, the identification of such cells was sought among a series of 19 asymptomatic carriers and 19 cases of tropical spastic paraparesis/HTLV-1 associated myelopathy (TSP/HAM) devoid of malignancy. Two PCR based protocols designed to amplify the host cell-HTLV-1 proviral integration sites were used. In all cases large numbers of proliferating clones could be identified. The proportion of some clones was > 1/1500 peripheral blood mononuclear cells (PBMCs) with the suggestion that their number increased as a function of age among asymptomatic carriers.
假设携带人类1型T细胞白血病病毒（HTLV-1）前病毒的T细胞克隆性扩增是HTLV-1感染的核心特征，那么在19名无症状携带者和19例无恶性肿瘤的热带痉挛性截瘫/HTLV-1相关脊髓病（TSP/HAM）患者中寻找此类细胞。使用了两种基于聚合酶链反应（PCR）的方案来扩增宿主细胞-HTLV-1前病毒整合位点。在所有病例中都能鉴定出大量增殖克隆。某些克隆在外周血单个核细胞（PBMC）中的比例>1/1500，提示其数量在无症状携带者中随年龄增加而增多。
