Brunel F, Zakin M M, Buc H, Buckle M
Unité d'Expression des Gènes Eucaryotes, Institut Pasteur, Paris, France.
Nucleic Acids Res. 1996 May 1;24(9):1608-15. doi: 10.1093/nar/24.9.1608.
Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.
多嘧啶序列结合（PTB）蛋白是一种功能未知的细胞因子。各种RNA或单链DNA序列已被证明可与PTB相互作用。在本文中，我们使用激光紫外交联和电泳迁移率变动分析来探测DNA-蛋白质相互作用，证明PTB在单链DNA靶标上的结合具有高度序列特异性。我们提供的数据表明，PTB与腺病毒主要晚期启动子转录起始位点的顶链相互作用，该序列富含嘧啶残基。我们还证明，PTB被组织成至少两个不同的结合结构域。
