毛细管电泳-电喷雾电离质谱法在分子多样性测定中的应用。
Application of capillary electrophoresis-electrospray ionization mass spectometry in the determination of molecular diversity.
作者信息
Dunayevskiy Y M, Vouros P, Wintner E A, Shipps G W, Carell T, Rebek J
机构信息
Department of Chemistry, Barnett Institute, Northeastern University, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6152-7. doi: 10.1073/pnas.93.12.6152.
Abstract
By means of capillary electrophoresis coupled online to electrospray ionization MS, a library of theoretically 171 disubstituted xanthene derivatives was analyzed. The method allowed the purity and makeup of the library to be determined: 160 of the expected compounds were found to be present, and 12 side-products were also detected in the mixture. Due to the ability of capillary electrophoresis to separate analytes on the basis of charge, most of the xanthene derivatives could be resolved by simple capillary electrophoresis-MS procedures even though 124 of the 171 theoretical compounds were isobaric with at least one other molecule in the mixture. Any remaining unresolved peaks were resolved by MS/MS experiments. The method shows promise for the analysis of small combinatorial libraries with fewer than 1000 components.
摘要
通过将毛细管电泳与电喷雾电离质谱在线联用，对一个理论上包含171种二取代呫吨衍生物的文库进行了分析。该方法能够确定文库的纯度和组成：发现混合物中存在160种预期化合物，还检测到12种副产物。由于毛细管电泳能够根据电荷分离分析物，即使171种理论化合物中的124种与混合物中的至少一种其他分子具有相同的分子量，大多数呫吨衍生物也可以通过简单的毛细管电泳 - 质谱程序进行分离。任何剩余的未分离峰通过串联质谱实验得以分离。该方法在分析少于1000个组分的小型组合文库方面显示出前景。
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